09689

Sigma-Aldrich

Ammonium acetate

BioUltra, for molecular biology, ≥99.0%

Linear Formula:
CH3CO2NH4
CAS Number:
Molecular Weight:
77.08
Beilstein/REAXYS Number:
4186741
EC Number:
MDL number:
eCl@ss:
39021908
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

product line

BioUltra

assay

≥99.0% (calc. based on dry substance, T)
≥99.0%

form

solid

impurities

insoluble matter, passes filter test
≤0.005% KMnO4-reducing substances
≤2% water

ign. residue

≤0.01% (as SO4)

pH

6.5-7.5 (25 °C, 1 M in H2O)

mp

110-112 °C (dec.) (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5 mg/kg
nitrate (NO3-): ≤10 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤2 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.015
λ: 280 nm Amax: ≤0.010

SMILES string

N.CC(O)=O

InChI

1S/C2H4O2.H3N/c1-2(3)4;/h1H3,(H,3,4);1H3

InChI key

USFZMSVCRYTOJT-UHFFFAOYSA-N

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Related Categories

Application

Ammonium acetate can be used to study x-ray crystallography, optimization reagents, protein structural analysis and proteomics. Ammonium acetate has been used in a study to develop a novel and simple high-performance liquid chromatography method for the simultaneous determination of two selective serotonin reuptake inhibitors and two serotonin-norepinephrine reuptake inhibitors in alternative samples of toxicological interest such as hair, nail clippings, and cerebrospinal fluid.

Other Notes

Elution buffer in separation of proteins by gel filtration, ammonium acetate is volatile and can be removed during lyophilization.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

C.W. Hirs
Methods in Enzymology, 47, 105-105 (1977)
Hong Lin et al.
Journal of the American Society for Mass Spectrometry, 23(12), 2122-2131 (2012-09-21)
Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic...
Jessica Z Bereszczak et al.
Protein science : a publication of the Protein Society, 23(7), 884-896 (2014-04-10)
Hepatitis B virus core-antigen (capsid protein) and e-antigen (an immune regulator) have almost complete sequence identity, yet the dimeric proteins (termed Cp149d and Cp(-10)149d , respectively) adopt quite distinct quaternary structures. Here we use hydrogen deuterium exchange-mass spectrometry (HDX-MS) to...
Elena N Kitova et al.
Journal of the American Society for Mass Spectrometry, 25(11), 1908-1916 (2014-08-20)
A semiquantitative electrospray ionization mass spectrometry (ESI-MS) binding assay suitable for analyzing mixtures of oligosaccharides, at unknown concentrations, for interactions with target proteins is described. The assay relies on the differences in the ratio of the relative abundances of the...
Xumin Zhang et al.
Analytical chemistry, 84(18), 7785-7792 (2012-08-15)
Untargeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics studies are usually carried out in both positive and negative ion modes; however, it is frequently ignored that the optimal conditions in positive ion mode and negative ion mode are often not the same....

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