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09743

Supelco

3,3′,5,5′-Tetramethylbenzidine

standard for GC

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

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250 MG
$37.60

$37.60

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250 MG
$37.60

About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
54827-17-7
Molecular Weight:
240.34
Beilstein/REAXYS Number:
2808541
EC Number:
259-364-6
MDL number:
MFCD00007748
UNSPSC Code:
41116107
PubChem Substance ID:
329748813
NACRES:
NA.24

$37.60

Available to ship onMay 20, 2025Details

Request a Bulk Order

grade

analytical standard

Quality Level

100

assay

≥99.0% (GC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

166-171 °C
168-171 °C (lit.)

application(s)

environmental

format

neat

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples[1] and dextromethorphan and its major metabolite dextrorphan in urine samples[2] using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).

Other Notes

this product will be discontinued, please find the alternative product here :
860336
Standard for determining aromatic amines released from azo-dyes in commodities[3]

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Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK9666
BCCG9352
BCCD6503
BCCC9345
BCBV5022

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Development and validation of ELISA and GC-MS procedures for the quantification of dextromethorphan and its main metabolite dextrorphan in urine and oral fluid
Rodrigues CW, et al.
Journal of Analytical Toxicology, 32(3), 220-226 (2008)
G. Rohm et al.
GIT Labor-Fachzeit., 1080-1080 (1997)
Residue analysis of the pharmaceutical diclofenac in different water types using ELISA and GC?MS
Deng A, et al.
Environmental Science & Technology, 37(15), 3422-3429 (2003)
Michael F Sharp et al.
Molecular immunology, 62(1), 77-85 (2014-06-29)
Fish allergy is a common food allergy, with prevalence rates in the general population ranging between 0.2% and 2.3%. In both adults and children fish ranks in the top eight foods known to cause IgE mediated food allergy. Fish allergy
Per A Larsson et al.
Colloids and surfaces. B, Biointerfaces, 101, 205-209 (2012-09-27)
A new method for detection of viruses has been developed. The entire assay can be performed within 2h, and consists of a polyelectrolyte-multilayer-modified cellulosic filter paper combined with immunodetection. The M13 bacteriophage was used as a model virus. A visual
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