09743

Supelco

3,3′,5,5′-Tetramethylbenzidine

standard for GC

Synonym(s):
TMB substrate, TMB, Sure Blue TMB, BM blue, TMB Blotting Plus
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
Molecular Weight:
240.34
Beilstein/REAXYS Number:
2808541
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.24

Quality Level

grade

analytical standard
standard for GC

assay

≥99.0% (GC)

shelf life

limited shelf life, expiry date on the label

application(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

166-171 °C
168-171 °C (lit.)

Featured Industry

Environmental

format

neat

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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Application

3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples and dextromethorphan and its major metabolite dextrorphan in urine samples using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Other Notes

this product will be discontinued, please find the alternative product here :
860336
Standard for determining aromatic amines released from azo-dyes in commodities

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

G. Rohm et al.
GIT Labor-Fachzeit., 1080-1080 (1997)
Residue analysis of the pharmaceutical diclofenac in different water types using ELISA and GC?MS
Deng A, et al.
Environmental Science & Technology, 37(15), 3422-3429 (2003)
Development and validation of ELISA and GC-MS procedures for the quantification of dextromethorphan and its main metabolite dextrorphan in urine and oral fluid
Rodrigues CW, et al.
Journal of Analytical Toxicology, 32(3), 220-226 (2008)
Ronit Mazor et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(23), 8571-8576 (2014-05-07)
Nonhuman proteins have valuable therapeutic properties, but their efficacy is limited by neutralizing antibodies. Recombinant immunotoxins (RITs) are potent anticancer agents that have produced many complete remissions in leukemia, but immunogenicity limits the number of doses that can be given...
Michael F Sharp et al.
Molecular immunology, 62(1), 77-85 (2014-06-29)
Fish allergy is a common food allergy, with prevalence rates in the general population ranging between 0.2% and 2.3%. In both adults and children fish ranks in the top eight foods known to cause IgE mediated food allergy. Fish allergy...

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