09743

3,3′,5,5′-Tetramethylbenzidine

standard for GC

• Synonym(s): BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
• Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂
• CAS Number: 54827-17-7
• Molecular Weight: 240.34
• Beilstein: 2808541
• EC Number: 259-364-6
• MDL number: MFCD00007748
• PubChem Substance ID: 329748813
• NACRES: NA.24

Properties

• grade: analytical standard; standard for GC
• Quality Level: 100
• Assay: ≥99.0% (GC)
• shelf life: limited shelf life, expiry date on the label
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• mp: 166-171 °C; 168-171 °C (lit.)
• application(s): environmental
• format: neat
• SMILES string: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples and dextromethorphan and its major metabolite dextrorphan in urine samples using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Other Notes

this product will be discontinued, please find the alternative product here :
860336

Standard for determining aromatic amines released from azo-dyes in commodities

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H302 - H351 - H413
• Precautionary Statements: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves