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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

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Synonym(s):
3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
Empirical Formula (Hill Notation):
C19H10Br4O5S
CAS Number:
Molecular Weight:
669.96
Beilstein/REAXYS Number:
61698
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

ACS reagent

Quality Level

form

powder

technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

mp

273 °C (lit.)

solubility

ethanol: 0.1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

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This Item
B01261.08122318744
Bromophenol Blue ACS reagent

114391

Bromophenol Blue

Bromophenol Blue titration: suitable

B0126

Bromophenol Blue

Bromophenol blue indicator ACS,Reag. Ph Eur

1.08122

Bromophenol blue

Bromophenol Blue solution 0.04 wt. % in H2O

318744

Bromophenol Blue solution

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

technique(s)

titration: suitable

form

powder

form

powder

form

solid

form

liquid

solubility

ethanol: 0.1%

solubility

methanol: 10 mg/mL

solubility

-

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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