MilliporeSigma
All Photos(4)

114413

Sigma-Aldrich

Bromothymol Blue

ACS reagent, Dye content 95 %

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
3′,3″-Dibromothymolsulfonphthalein
Empirical Formula (Hill Notation):
C27H28Br2O5S
CAS Number:
Molecular Weight:
624.38
Beilstein:
373934
EC Number:
MDL number:
PubChem Substance ID:

grade

ACS reagent

Quality Level

form

powder

composition

Dye content, 95%

visual transition interval

6.0-7.6, yellow to blue(passes test)

mp

200-202 °C (lit.)

λmax

420 nm

ε (extinction coefficient)

≥17000 at 419-425 nm in methanol
≥8000 at 326-332 nm in methanol
≥9000 at 279-285 nm in methanol

storage temp.

room temp

SMILES string

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

InChI key

NUHCTOLBWMJMLX-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
B36761.03026510998
Bromothymol Blue ACS reagent, Dye content 95 %

Sigma-Aldrich

114413

Bromothymol Blue

B.T.B. lactose agar suitable for microbiology, NutriSelect® Plus

Millipore

B3676

B.T.B. lactose agar

Bromothymol blue indicator ACS,Reag. Ph Eur

Supelco

1.03026

Bromothymol blue

form

powder

form

powder

form

solid

form

powder

composition

Dye content, 95%

composition

-

composition

-

composition

Dye content, 95%

mp

200-202 °C (lit.)

mp

-

mp

-

mp

230 °C (dec.) (lit.)

λmax

420 nm

λmax

-

λmax

-

λmax

590 nm

ε (extinction coefficient)

≥17000 at 419-425 nm in methanol, ≥8000 at 326-332 nm in methanol, ≥9000 at 279-285 nm in methanol

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

≥15000 at 306-310 nm in 0.1 M NaOH at 0.01 g/L, ≥8000 at 378-382 nm in 0.1 M NaOH at 0.01 g/L

General description

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
  • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
  • for the demonstration of fungal hyphae within plant roots
  • in the assessment of the soluble acidity within single rice grains
  • use in an agar gel medium for enumeration of Bacillus cereus
  • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Methyl Orange ACS reagent, Dye content 85 %

Sigma-Aldrich

114510

Methyl Orange

Bromocresol Purple BioReagent, suitable for indicator, Dye content 90 %

Sigma-Aldrich

114375

Bromocresol Purple

Reza Amin et al.
Micromachines, 11(6) (2020-07-01)
To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic
Joseph A Price
Journal of biochemical and biophysical methods, 70(3), 441-444 (2006-12-16)
Phospholipase A(2) is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is
Bartlomiej Kowalczyk et al.
Nanoscale, 2(11), 2366-2369 (2010-10-13)
Films comprising metal nanoparticles are assembled on the surfaces of liquid droplets of different shapes and macroscopic dimensions. These films are reinforced by dithiol crosslinks and are mechanically rugged yet permeable to the diffusion of small molecules.
Kristina Jurcic et al.
Journal of chromatography. A, 1134(1-2), 317-325 (2006-10-07)
An effective sample preconcentration technique for proteins and peptides was recently developed using capillary electrophoresis (CE) with discontinuous buffers [C.A. Nesbitt, J.T.-M. Lo, K.K.-C. Yeung, J. Chromatogr. A 1073 (2005) 175]. Two buffers of different pH created a junction to
Bambang Kuswandi et al.
Talanta, 74(4), 613-618 (2008-03-29)
An optical fiber biosensor consisting of acetylcholinesterase (AChE) and bromothymol blue (BTB) doped sol-gel film was employed to detect organophosphate pesticide chlorpyrifos. The main advantage of this optical biosensor is the use of a single sol-gel film with immobilized AChE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service