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114545

Sigma-Aldrich

Thymol Blue

ACS reagent

Synonym(s):
Thymolsulfonphthalein
Empirical Formula (Hill Notation):
C27H30O5S
CAS Number:
Molecular Weight:
466.59
Beilstein:
368036
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

ACS reagent

Quality Level

form

powder

clarity of soln

passes test

technique(s)

titration: suitable

visual transition interval

1.2-2.8, red to yellow(acid range)
1.2-2.8, red to yellow
8.0-9.2, yellow to blue(alkaline range)
8.0-9.2, yellow to blue

mp

221-224 °C (dec.) (lit.)

solubility

ethanol: 0.1%, clear

density

0.979 g/cm3

λmax

376 nm (2nd)
594 nm

ε (extinction coefficient)

≥12000 at 298-302 nm in 0.1 M NaOH at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(C)c1cc(c(C)cc1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)cc4C

InChI

1S/C27H30O5S/c1-15(2)19-13-22(17(5)11-24(19)28)27(21-9-7-8-10-26(21)33(30,31)32-27)23-14-20(16(3)4)25(29)12-18(23)6/h7-16,28-29H,1-6H3

InChI key

PRZSXZWFJHEZBJ-UHFFFAOYSA-N

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This Item
861367952901.08176
Thymol Blue ACS reagent

Sigma-Aldrich

114545

Thymol Blue

Methyl Blue for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

Sigma-Aldrich

95290

Methyl Blue

Thymol blue indicator ACS, Reag. Ph Eur

Supelco

1.08176

Thymol blue

form

powder

form

powder or crystals

form

powder or crystals

form

solid

technique(s)

titration: suitable

technique(s)

-

technique(s)

-

technique(s)

-

mp

221-224 °C (dec.) (lit.)

mp

283-285 °C (dec.) (lit.)

mp

-

mp

221 °C (decomposition)

solubility

ethanol: 0.1%, clear

solubility

-

solubility

water: 0.01 g/10 mL, blue to very deep blue

solubility

-

density

0.979 g/cm3

density

-

density

-

density

-

General description

Thymol Blue is a sulfonephthalein dye. It is also known as thymolsulfonphthalein.

Application

Thymol Blue has been used for the in situ spectrophotometric pH measurement.

Biochem/physiol Actions

Thymol Blue is a pH indicator, which is yellow/orange at low pH and blue in color at high pH.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Global stability for convection induced by absorption of radiation.
Straughan B
Dynamics of atmospheres and oceans, 35, 351-361 (2002)
Douglas R Macfarlane et al.
Chemical communications (Cambridge, England), 46(41), 7703-7705 (2010-09-28)
We describe hydrated ionic liquids which have an intrinsic proton buffering action and hence an ability to control proton activity in an ionic medium.
S Passamonti et al.
Biochimica et biophysica acta, 943(2), 119-125 (1988-08-18)
Sulfobromophthalein electrogenic uptake into rat liver plasma membrane vesicles was shown to admit only the quinoid, trivalent anion. The minimum requirement for this electrogenic process has been investigated in rat liver plasma membrane vesicles by using Thymol blue, a pH-indicator
N Takai et al.
Artificial organs, 15(2), 86-89 (1991-04-01)
An optical fiber sensor utilizing Thymol blue and an ion-exchange resin complex in a cellulose acetate membrane was developed. By monitoring several different chromophores of Thymol blue, the sensor could measure the pH of the solution from 1.0 to 12.0
Ali A Ensafi et al.
Journal of hazardous materials, 148(1-2), 319-325 (2007-03-30)
An on-line flow system was used to develop a selective and efficient on-line sorbent extraction preconcentration system for cadmium. The method is based on adsorption of cadmium ions onto the activated carbon modified with methyl thymol blue. Then the adsorbed

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