MilliporeSigma
All Photos(3)

131164

Sigma-Aldrich

Indigo carmine

certified by the Biological Stain Commission, Dye content 85 %

Synonym(s):
Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine
Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
Molecular Weight:
466.35
Colour Index Number:
73015
Beilstein:
4103904
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

Assay

>85.0% (HPLC)

form

solid

composition

Dye content, 85%

pH

3.87 (30 °C, 7.14 g/L)

mp

≥340 °C

density

0.71 g/cm3 at 29 °C

λmax

608 nm

εmax

20,000 at 250-256 nm in water at 0.01 g/L
29500 at 284-290 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

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This Item
570001.04724C6152
Indigo carmine certified by the Biological Stain Commission, Dye content 85 %

Sigma-Aldrich

131164

Indigo carmine

Indigo carmine for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

Sigma-Aldrich

57000

Indigo carmine

Carmine certified by the Biological Stain Commission

Sigma-Aldrich

C6152

Carmine

grade

certified by the Biological Stain Commission

grade

for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)

grade

-

grade

certified by the Biological Stain Commission

assay

>85.0% (HPLC)

assay

-

assay

-

assay

-

form

solid

form

powder

form

-

form

powder

composition

Dye content, 85%

composition

-

composition

dye content, ≥80% spectrophotometric assay

composition

Dye content: minimum has not been established

pH

3.87 (30 °C, 7.14 g/L)

pH

3.87 (30 °C, 7.14 g/L)

pH

7 (20 °C, 10 g/L in H2O)

pH

-

mp

≥340 °C

mp

>340 °C

mp

>340 °C

mp

-

Application

Indigo carmine has been used to investigate its effects on ozone production by neutrophils.

Biochem/physiol Actions

Indigo Carmine (IC), commonly known as acid blue 74 or food blue 1 or FD and C blue 2 is a blue synthetic dye. It is used as a redox indicator in analytical chemistry and as a microscopic stain in biology. IC is also employed as a photometric detector. IC is a highly toxic indigoid dye and is associated with various health hazards such as irritation to the gastrointestinal tract leading to nausea, vomiting, and diarrhea and irritation in the respiratory tract leading to coughing and shortness of breath. IC exposure might also cause skin and eye irritations to humans. IC dye spray is used for the diagnosis of dysplasia in ulcerative colitis and is considered to be a simple, feasible, and safe method.

Suitability

Used as a collagen stain and in procedures using red nuclear stains.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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