MilliporeSigma
All Photos(3)

131164

Sigma-Aldrich

Indigo carmine

certified by the Biological Stain Commission, Dye content 85 %

Synonym(s):
Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine, Acid Blue 74
Empirical Formula (Hill Notation):
C16H8N2Na2O8S2
CAS Number:
Molecular Weight:
466.35
Colour Index Number:
73015
Beilstein:
4103904
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

assay

>85.0% (HPLC)

form

solid

composition

Dye content, 85%

pH

3.87 (30 °C, 7.14 g/L)

mp

≥340 °C

density

0.71 g/cm3 at 29 °C

λmax

608 nm

εmax

20,000 at 250-256 nm in water at 0.01 g/L
29500 at 284-290 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]S(=O)(=O)c1ccc2NC(\C(=O)c2c1)=C3\Nc4ccc(cc4C3=O)S([O-])(=O)=O

InChI

1S/C16H10N2O8S2.2Na/c19-15-9-5-7(27(21,22)23)1-3-11(9)17-13(15)14-16(20)10-6-8(28(24,25)26)2-4-12(10)18-14;;/h1-6,17-18H,(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b14-13+;;

InChI key

KHLVKKOJDHCJMG-QDBORUFSSA-L

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Application

Indigo carmine has been used to investigate its effects on ozone production by neutrophils.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Indigo Carmine (IC), commonly known as acid blue 74 or food blue 1 or FD and C blue 2 is a blue synthetic dye. It is used as a redox indicator in analytical chemistry and as a microscopic stain in biology. IC is also employed as a photometric detector. IC is a highly toxic indigoid dye and is associated with various health hazards such as irritation to the gastrointestinal tract leading to nausea, vomiting, and diarrhea and irritation in the respiratory tract leading to coughing and shortness of breath. IC exposure might also cause skin and eye irritations to humans. IC dye spray is used for the diagnosis of dysplasia in ulcerative colitis and is considered to be a simple, feasible, and safe method.

Suitability

Used as a collagen stain and in procedures using red nuclear stains.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Superoxide converts indigo carmine to isatin sulfonic acid: implications for the hypothesis that neutrophils produce ozone.
Kettle AJ
The Journal of Biological Chemistry, 279(18), 18521-18525 (2004)
An experimental study of indigo carmine removal from aqueous solution by electrocoagulation
Secula M S
Desalination, 277(1-3), 227-235 (2011)
J. Ind. Chem. Soc., 63, 533-533 (1986)
Pancolonic indigo carmine dye spraying for the detection of dysplasia in ulcerative colitis
Rutter M D
Gut, 53(2), 256-260 (2004)
Rice husk ash as an effective adsorbent: evaluation of adsorptive characteristics for Indigo Carmine dye.
Lakshmi UR
Journal of Environmental Management, 90(2), 710-720 (2009)

Protocols

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

Separation of Tartrazine; Amaranth; Indigo carmine; New Coccine; Sunset Yellow FCF; Allura Red AC; Fast Green FCF; Erioglaucine disodium salt; Erythrosin B sodium salt; Phloxine B; Rose bengal

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