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141194

Ponceau S

Dye content 75 %

Synonym(s):

3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt

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About This Item

Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
6226-79-5
Molecular Weight:
760.57
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24848493
EC Number:
228-319-2
Colour Index Number:
27195
MDL number:
MFCD00003892

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InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

form

powder

composition

Dye content, 75%

technique(s)

microbe id | staining: suitable

λmax

352 nm, 520 nm (2nd)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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1 of 4

This Item
P35041.04238P7170
Ponceau S Dye content 75 %

Sigma-Aldrich

141194

Ponceau S

Ponceau S BioReagent, suitable for electrophoresis

Sigma-Aldrich

P3504

Ponceau S

Ponceau S (C.I. 27195)

Sigma-Aldrich

1.04238

Ponceau S (C.I. 27195)

Ponceau S solution BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

Sigma-Aldrich

P7170

Ponceau S solution

technique(s)

microbe id | staining: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

western blot: suitable

form

powder

form

powder

form

solid

form

liquid

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

composition

Dye content, 75%

composition

-

composition

Dye, ≥80% spectrophotometric assay

composition

-

storage temp.

room temp

storage temp.

room temp

storage temp.

2-30°C

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Ponceau S (C.l. 27195) is an anionic, very hydrophilic disazo dye. It is also known as fast ponceau 2B.

Application

Ponceau S is widely used to stain proteins on gel electropherograms. It is a suitable stain for proteins adsorbed onto nitrocellulose membranes. It also has applications in botanical staining manuals as a stain for sieve plate elements.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7518
SHBS5342
SHBR9451
0000244684
0000244684

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Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
Aebersold RH
Proceedings of the National Academy of Sciences of the USA, 84(20), 6970-6974 (1987)
An intramembrane modulator of the ErbB2 receptor tyrosine kinase that potentiates neuregulin signaling.
Carraway KL III
The Journal of Biological Chemistry, 274(9), 5263-5266 (1999)
Expression of cyclooxygenase-2 (COX-2) in human invasive transitional cell carcinoma (TCC) of the urinary bladder.
Mohammed SI
Cancer Research, 59(22), 5647-5650 (1999)
Sarah Nicklas et al.
PloS one, 7(1), e30445-e30445 (2012-02-03)
Limb girdle muscular dystrophy type 2H (LGMD2H) is an inherited autosomal recessive disease of skeletal muscle caused by a mutation in the TRIM32 gene. Currently its pathogenesis is entirely unclear. Typically the regeneration process of adult skeletal muscle during growth
T G van Kooten et al.
Journal of biomedical materials research, 54(3), 385-389 (2001-02-24)
Research of cell-biomaterial interactions is building on knowledge and methods available in cell and molecular biology. Western blotting is one of the options to characterize protein expression in cell populations. Method transfer to biomaterial model systems is not trivial because
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