15222

N,O-Bis(trimethylsilyl)trifluoroacetamide

for GC derivatization, LiChropur^™, ≥99.0%

• Synonym(s): BSTFA
• Linear Formula: CF₃C[=NSi(CH₃)₃]OSi(CH₃)₃
• CAS Number: 25561-30-2
• Molecular Weight: 257.40
• Beilstein: 2050024
• EC Number: 247-103-9
• MDL number: MFCD00008269
• PubChem Substance ID: 329750779
• NACRES: NA.22

Properties

• grade: for GC derivatization
• Quality Level: 100
• Assay: ≥99.0% (GC); ≥99.0%
• form: liquid
• quality: LiChropur^™
• reaction suitability: reagent type: derivatization reagent; reaction type: Silylations
• technique(s): gas chromatography (GC): suitable
• refractive index: n20/D 1.384 (lit.); n20/D 1.384
• bp: 45-50 °C/14 mmHg (lit.)
• density: 0.969 g/mL at 25 °C (lit.)
• SMILES string: C[Si](C)(C)O\C(=N\[Si](C)(C)C)C(F)(F)F
• InChI: 1S/C8H18F3NOSi2/c1-14(2,3)12-7(8(9,10)11)13-15(4,5)6/h1-6H3/b12-7+
• InChI key: XCOBLONWWXQEBS-KPKJPENVSA-N

Description

General description

N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) is an excellent silylation reagent. For some sterically hindered primary or secondary nitro compounds, N,O-bis(trimethylsilyl)acetamide and BSTFA achieve better results than normal silylation conditions. BSTFA finds application in derivatizing amines. It is suitable for silyaltion of carboxylic acids, phenols, ureas, amides and alcohols.

Application

Learn more in the Product Information

BSTFA + 1% TMCS (trimethylsilyl) in ethyl acetate, acetonitrile and dichloromethane solvents may be used for silylation of steroid hormone 17a-ethinylestradiol (EE2) using gas chromatography-mass spectrometry (GC-MS). It may also be used for silylation of endogenous 2-monoglyceride, present in canine gut that binds to cannabinoid receptors.

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Warning
• Hazard Statements: H226 - H315 - H319
• Precautionary Statements: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 93.2 °F
• Flash Point(C): 34 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves