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for GC derivatization, LiChropur, 93.0-97.0% (GC)

Bis(trimethylsilyl)acetamide + Trimethylchlorosilane
PubChem Substance ID:

Quality Level


for GC derivatization


3.0-5.0% (trimethylchlorosilane, minor component, GC)
93.0-97.0% (GC)




trimethylchlorosilane (minor component), 3.0-5.0% GC

reaction suitability

reagent type: derivatization reagent
reaction type: Silylations


GC/MS: suitable
gas chromatography (GC): suitable

General description

BSA+TMCS combination, is one of the most commonly used silylating reagents. BSA is readily used for silylating a wide range of functional groups such as non-sterically hindered alcohols, amides, amines, amino acids, carboxylic acids, and enols. TMCS is a silylation catalyst always used with other silylation reagents to increase the reactivity in derivatization of alcohols, alkaloids, amines, biogenic amines, carboxylic acids, phenols, and steroids.


Learn more in the Product Information
Suitable for the derivatization of α-ketoacids, amines, amino acids, cycloserine, histamine and metabolites, phenylethylamines, phosphatidylserines and tryptamines. BSA+TMCS may be used as a derivatizing reagent for the determination of steryl glycosides in biodiesel using gas chromatography–mass spectrometry.
Suitable for the derivatization of a-ketoacids, amines, amino acids, cycloserine, histamine and metabolites, phenylethylamines, phosphatidylserines and tryptamines.

Features and Benefits

  • BSA+TMCS has good solvent properties and can function as a silylation reagent without additional solvents.        
  • Alternatively, the mixture is very soluble in most commonly used silylation solvents.        
  • This combination is extremely sensitive to moisture and should be handled under dry conditions.

Other Notes

Reagent for trimethylsilyl diglyceride, trimethylsilyl (TMS) and trimethylsilyl oximes.
Powerful silylation mixture with wide applicability

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Signal Word


Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Flam. Liq. 2 - Skin Corr. 1A

Supplementary Hazards

Storage Class Code

3 - Flammable liquids

WGK Germany


Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

J. Heberle et al.
Silylating Agents, 2nd ed. (1995)
Laura Navarro Cantó et al.
Frontiers in neurology, 10, 1157-1157 (2019-11-19)
Background and objective: Chronic relapsing inflammatory optic neuritis (CRION) is one of the more common phenotypes related to myelin oligodendrocyte glycoprotein antibodies (MOG-Abs). The absence of specific biomarkers makes distinguishing between CRION and relapsing inflammatory ON (RION) difficult. A recent...
P. Englmaier
Z. Anal. Chem., 324, 338-338 (1986)
Ashwin Unnikrishnan et al.
Nucleic acids research, 44(22), 10644-10661 (2016-09-09)
Aberrant stem cell-like gene regulatory networks are a feature of leukaemogenesis. The ETS-related gene (ERG), an important regulator of normal haematopoiesis, is also highly expressed in T-ALL and acute myeloid leukaemia (AML). However, the transcriptional regulation of ERG in leukaemic...
Victor Crivianu-Gaita et al.
Biosensors & bioelectronics, 78, 92-99 (2015-11-26)
There are currently no biosensors that are able to reliably detect the process of cancer metastasis. We describe the first label-free real-time ultra-high frequency acoustic wave biosensor prototype capable of detecting the breast and prostate cancer metastasis biomarker, parathyroid hormone-related...


Silylation of Non-Steroidal Anti-Inflammatory Drugs

Results of a study involving the ability few Fluka silylating reagents to form GC-MS-compatible trimethylsilylmethyl derivatives of NSAIDs

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