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15604

Sigma-Aldrich

Urea

puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

Synonym(s):
Carbonyldiamide, Carbamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

grade

puriss.

assay

99.0-100.5%
99.0-101.0% (calc. on dry substance)

form

solid

quality

meets analytical specification of Ph. Eur., BP, USP

impurities

alkalic reac. substances, complies
≤0.001% heavy metals (as Pb)
≤0.04% insoluble in ethanol
≤0.05% ammonium (NH4)
≤0.1% biuret(carbamylurea)

ign. residue

≤0.1% (as SO4)

loss

≤1.0% loss on drying, 105 °C, 1 h

mp

132-135 °C (lit.)
132-135 °C

solubility

H2O: soluble 480 g/L at 20 °C

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Fe: ≤0.5 mg/kg

suitability

in accordance for appearance of solution
in accordance for identity

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

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General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.
Urea can be prepared from ammonia and carbon-dioxide, via Haber-Bosch process. It can also be used to prepare urea-formaldehyde resins, via condensation reaction.

Application

Urea has been used in the following studies:
  • Synthesis of SnO2 nanoparticles.
  • Synthesis of urethanes, ureides and semicarbazides.
  • Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain
  • Protein electrophoretic studies.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Braun D, et al. et al.
Polymer Synthesis: Theory and Practice: Fundamentals, Methods, Experiments ; with 31 Tables (2001)
Nova I and Tronconi E et al.
Urea-SCR Technology for deNOx after treatment of Diesel Exhausts (2014)
Genna L Andrews et al.
Analytical chemistry, 83(13), 5442-5446 (2011-05-31)
The TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth. Employing a Saccharomyces...
Joost P J J Hegmans et al.
The American journal of pathology, 164(5), 1807-1815 (2004-04-28)
Exosomes are small membrane vesicles secreted into the extracellular compartment by exocytosis. Tumor exosomes may be involved in the sampling of antigens to antigen presenting cells or as decoys allowing the tumor to escape immune-directed destruction. The proteins present in...
Eagleson M.
Concise Encyclopedia Chemistry, 1137-1137 (1994)

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