MilliporeSigma
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17171

Millipore

Mineral modified Glutamate Broth (Base)

suitable for microbiology, NutriSelect® Plus

NACRES:
NA.85

Agency

according to ISO 16649-3:2015

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

L-arginine, 0.04 g/L
L-aspartic acid, 0.048 g/L
bromocresol purple, 0.02 g/L
calcium chloride, 0.02 g/L
L-cystine, 0.04 g/L
dipotassium phosphate, 1.8 g/L
ferric ammonium citrate, 0.02 g/L
lactose, 20 g/L
magnesium sulfate, 0.2 g/L
nicotinic acid, 0.002 g/L
pantothenic acid, 0.002 g/L
sodium formate, 0.5 g/L
thiamine, 0.002 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.7±0.2 (25 °C)

application(s)

agriculture
agriculture
environmental
food and beverages
water monitoring

microbiology

suitability

nonselective and differential for Escherichia coli
nonselective and differential for coliforms
nonselective and differential for enterobacteriaceae

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Mucate Broth suitable for microbiology, NutriSelect® Plus

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SD Agar suitable for microbiology, NutriSelect® Basic

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sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

limited shelf life, expiry date on the label

composition

L-arginine, 0.04 g/L , L-aspartic acid, 0.048 g/L , bromocresol purple, 0.02 g/L , calcium chloride, 0.02 g/L , L-cystine, 0.04 g/L , dipotassium phosphate, 1.8 g/L , ferric ammonium citrate, 0.02 g/L , lactose, 20 g/L , magnesium sulfate, 0.2 g/L , nicotinic acid, 0.002 g/L , pantothenic acid, 0.002 g/L , sodium formate, 0.5 g/L , thiamine, 0.002 g/L

composition

bromothymol blue, 0.024 g/L , mucic acid, 10 g/L , peptic digest of animal tissue, 10 g/L

composition

agar, 20 g/L , ammonium sulfate, 5 g/L , biotin, 0.02 mg/L , boric acid, 0.5 mg/L , calcium chloride, 0.1 g/L , calcium pantothenate, 2 mg/L , copper sulfate, 0.04 mg/L , dextrose, 20 g/L , dipotassium hydrogen phosphate, 0.15 g/L , ferric chloride, 0.2 mg/L , folic acid, 0.002 mg/L , inositol, 10 mg/L , magnesium sulfate, 0.5 g/L , manganese sulfate, 0.4 mg/L , niacin, 0.4 mg/L , PABA, 0.2 mg/L , potassium dihydrogen phosphate, 0.85 g/L , potassium iodide, 0.1 mg/L , pyridoxine HCl, 0.4 mg/L , riboflavin, 0.2 mg/L , sodium chloride, 0.1 g/L , sodium molybdate, 0.2 mg/L , thiamine HCl, 0.4 mg/L , zinc sulfate, 0.4 mg/L

composition

agar, 13.0 g/L , bile salts, 1.5 g/L , crystal violet, 0.002 g/L , dextrose, 10.0 g/L , neutral red, 0.03 g/L , peptone (from gelatin), 7.0 g/L , sodium chloride, 5.0 g/L , yeast extract, 3.0 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

Application

For the enumeration of coliform bacteria in water and waste water samples.

Preparation Note

Suspend 22.7 g of Part A and 12.7 g of Part B in 1 litre distilled water containing 5 g of ammonium chloride. Heat is necessary to dissolve the medium completely. Dispense in tubes containing inverted Durham′s tubes. Sterilize by autoclaving at 115°C for 10 minutes. For single strength medium use 11.35 g of Part A and 6.35 g of Part B in 1 litre distilled water containing 2.5 g ammonium chloride.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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