MilliporeSigma
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17173

Millipore

Rappaport Vassiliadis Broth, modified

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Magnesium chloride Malachite Green Broth, Modified Rappaport Vassiliadis Broth, RV Broth, Salmonella Enrichment Broth acc. to Rappaport and Vassiliadis
NACRES:
NA.85

Agency

according to ISO 19250:2010
according to ISO 6579-1:2017

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.2±0.2 (25 °C)

application(s)

environmental
food and beverages
food and beverages
pathogen testing

microbiology

suitability

selective for Salmonella spp.

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This Item
597518991692322
sterility

non-sterile

sterility

-

sterility

non-sterile

sterility

non-sterile

form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

Application

Used as a selective enrichment medium for the isolation of Salmonella species from food and environmental specimens.

Components

Papaic digest of soyabean meal 5 g/1110 mL;
Sodium chloride 8 g/1110 mL;
Monopotassium phosphate 1.6 g/1110 mL;
Magnesium chloride anhydrous 18.7 g/l (is equivalent to 40 g/1110 mL Magnesium chloride hexahydrate);
Malachite Green 0.04 g/1110 ml

Preparation Note

Suspend 30.0 g in 1 litre distilled water. Heat gently if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 115°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

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