17185

m-Enterococcus Agar, modified

NutriSelect^® Plus, powder, selective and differential for Enterococcus spp., pack of 500 g, suitable for microbiology

• Synonym(s): Membrane filter Enterococcus Agar, modified
• NACRES: NA.85
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Plus
• Form: powder
• Suitability: selective and differential for Enterococcus spp.
• Application(s): agriculture; environmental; food and beverages; water monitoring, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L , cycloheximide, 0.05 g/L , esculin, 1 g/L , nalidixic acid, 0.25 g/L , pancreatic digest of gelatin, 10 g/L , sodium azide, 0.15 g/L , sodium chloride, 15 g/L , yeast extract, 30 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: greenish yellow
• final pH: 7.1±0.2 (25 °C)
• application(s): agriculture; environmental; food and beverages; water monitoring, microbiology
• suitability: selective and differential for Enterococcus spp.

Description

General description

m-EnterococcusAgar, modified, is a selective dehydrated culture medium formulated for thedetection and enumeration of enterococci, particularly in water analysis. Theformulation contains peptones, yeast extract, esculin, sodium azide, nalidixicacid, cycloheximide, and agar, providing a highly selective environment thatsupports the growth of enterococci while inhibiting a wide range of Gram‑negative bacteria, fungi,and many other competing floras. Pancreatic digest ofgelatin and yeast extract acts as a source of carbon, nitrogen, minerals,vitamins, and other essential nutrients for growth. Sodium chloride maintainsisotonic conditions of the medium, besides the provision of essential ions to avariety of organisms. Sodium azide, Cycloheximide, and Nalidixic acid inhibitmany species of bacteria and fungi and thus make the medium selective. Esculinis hydrolyzed by bacterial enzymes to esculetin and dextrose. TTC is reduced byEnterococci to insoluble formazan inside the bacterial cells, which gives redcoloration to colonies

Application

m-Enterococcus Agar, modified, is a selective medium for the enumeration and detection of enterococci in water and sewage with the membrane-filter technique and in the direct application procedure in food. It is intended for the recovery and enumeration of enterococci from water samples using the membrane filtration technique and is used in conjunction with Esculin Iron Agar for confirmation and identification of typical colonies. It is suitable for monitoring drinking water, recreational water, and other environmental water samples within microbiological water quality testing schemes where enterococci serve as indicators of fecal contamination. The selectivity provided by sodium azide and nalidixic acid allows for reliable recovery of enterococci even in samples with mixed flora, supporting quantitative assessments and regulatory compliance testing in environmental and public health laboratories.

Features and Benefits

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment.
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Suspend 71.4 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45°C and aseptically add 15 ml of sterile 1% TTC Solution Cat. No. No. 17779). Mix well and pour into sterile petri plates. Warning: Cycloheximide is very toxic. Avoid skin contact or aerosol formation and inhalation.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves