17215

WL Differential Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): WLD Agar, Wallerstein Differential Agar
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: actidione (cycloheximide), 0.004 g/L ; agar, 20 g/L ; bromocresol green, 0.022 g/L ; calcium chloride, 0.125 g/L ; casein enzymic hydrolysate, 5 g/L ; dextrose, 50 g/L ; ferric chloride, 0.0025 g/L ; magnesium sulfate, 0.125 g/L ; manganese sulfate, 0.0025 g/L ; monopotassium phosphate, 0.55 g/L ; potassium chloride, 0.425 g/L ; yeast extract, 4 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 5.5±0.2 (25 °C)
• application(s): bioburden testing; food and beverages; microbiology
• suitability: selective and differential for Escherichia coli; selective and differential for Lactobacillus spp.; selective and differential for Proteus spp.; selective and differential for bacteria (General Media); selective and differential for coliforms

Description

General description

WL (Wallerstein Laboratory) Differential Agar, modified is formulated as described by Green and Gray for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria. WL Differential Agar helps in selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations.

Application

WL Differential Agar has been used for the enumeration of Kluyveromyces marxianus culture.

Preparation Note

Suspend 80.26 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. If desired, to obtain a pH of 6.5, add 1% solution of sodium bicarbonate.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves