17215

Millipore

WL Differential Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):
WLD Agar, Wallerstein Differential Agar
NACRES:
NA.74

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

actidione (cycloheximide), 0.004 g/L
agar, 20 g/L
bromocresol green, 0.022 g/L
calcium chloride, 0.125 g/L
casein enzymic hydrolysate, 5 g/L
dextrose, 50 g/L
ferric chloride, 0.0025 g/L
magnesium sulfate, 0.125 g/L
manganese sulfate, 0.0025 g/L
monopotassium phosphate, 0.55 g/L
potassium chloride, 0.425 g/L
yeast extract, 4 g/L

manufacturer/tradename

NutriSelect® Plus

application(s)

bioburden testing: suitable
microbiological culture: suitable
microbiology: suitable

final pH

5.5±0.2 (25 °C)

Featured Industry

Food and Beverages

suitability

selective and differential for Escherichia coli
selective and differential for Lactobacillus spp.
selective and differential for Proteus spp.
selective and differential for bacteria (General Media)
selective and differential for coliforms

Related Categories

General description

WL (Wallerstein Laboratory) Differential Agar, modified is formulated as described by Green and Gray for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria. WL Differential Agar helps in selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations.

Application

WL Differential Agar has been used for the enumeration of Kluyveromyces marxianus culture.

Preparation Note

Suspend 80.26 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. If desired, to obtain a pH of 6.5, add 1% solution of sodium bicarbonate.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
A differential procedure for bacteriological studies useful in the fermentation industry
Green SR and Gray PP
Archives of Biochemistry and Biophysics, 32(1), 59-69 (1951)
Interactions between Kluyveromyces marxianus and Saccharomyces cerevisiae in tequila must type medium fermentation
Lopez CLF, et al.
World Journal of Microbiology & Biotechnology, 30(8), 2223-2229 (2014)
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