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17231

Millipore

SPS Agar, modified

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Sulfite Polymyxin Sulfadiazine Agar, modified
NACRES:
NA.74

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
casein enzymic hydrolysate, 15 g/L
ferric citrate, 0.5 g/L
polymyxin B sulfate, 0.01 g/L
polysorbate 80, 0.05 g/L
sodium sulfite, 0.5 g/L
sodium thioglycollate, 0.1 g/L
sulfadiazine, 0.12 g/L
yeast extract, 10 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

bioburden testing
food and beverages

microbiology

suitability

selective and differential for Clostridium spp.

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shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , casein enzymic hydrolysate, 15 g/L , ferric citrate, 0.5 g/L , polymyxin B sulfate, 0.01 g/L , polysorbate 80, 0.05 g/L , sodium sulfite, 0.5 g/L , sodium thioglycollate, 0.1 g/L , sulfadiazine, 0.12 g/L , yeast extract, 10 g/L

composition

agar, 14 g/L , ascorbic acid, 0.1 g/L , dipotassium phosphate, 0.01 g/L , ferrous ammonium sulfate, 0.1 g/L , magnesium sulfate, 0.2 g/L , sodium chloride, 10 g/L , yeast extract, 1 g/L

composition

agar, 15 g/L , bromocresol purple, 0.04 g/L , L-cysteine hydrochloride, 1 g/L , ferric chloride 6H2O, 0.09 g/L , indoxyl-β-D-glucoside, 0.06 g/L , magnesium sulfate 7H2O, 0.1 g/L , sucrose, 5 g/L , tryptose, 30 g/L , yeast extract, 20 g/L

composition

agar, 15 g/L , casein hydrolysate, 3.7 g/L , di-potassium hydrogen phosphate, 3.3 g/L , lactose, 9.4 g/L , pararosanilin (fuchsin), 0.8 g/L , peptone from meat, 3.7 g/L , potassium hydrogen phosphate, 1 g/L , sodium chloride, 3.7 g/L , sodium deoxycholate, 0.1 g/L , sodium lauryl sulfate, 0.05 g/L , sodium sulfite, 1.6 g/L , tryptose, 7.5 g/L , yeast extract, 1.2 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbe id | metabolite detection: suitable, microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

final pH

7.4±0.2 (25 °C)

final pH

7.6±0.2 (25 °C)

final pH

7.2±0.2±25 °C

Application

For the selective isolation and enumeration of Clostridium perfringens from foods.

Preparation Note

Suspend 41.28 g in 1 litre distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour in sterile petri plates containing inoculum. Allow to solidify and if desired, pour the cover layer using about 5 ml sterile medium. Incubate anaerobically.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridium perfringens can cause contamination in undercooked or improperly sterillized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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