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17975

Supelco

Ninhydrin reagent according to Stahl

for TLC derivatization

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About This Item

UNSPSC Code:
41116105
NACRES:
NA.22

grade

for TLC derivatization

Quality Level

100

form

liquid

technique(s)

thin layer chromatography (TLC): suitable

impurities

1-butanol
 acetic acid

density

0.823 g/mL at 20 °C

General description

Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product. Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.

Application

1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid
Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

93.2 °F - closed cup

flash_point_c

34 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF2980
BCBV2843

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Thin Layer Chromatography in Phytochemistry.
Hajnos WM
Science, 312-312 (2008)
M K Gaitonde
The Biochemical journal, 104(2), 627-633 (1967-08-01)
1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring
B Starcher
Analytical biochemistry, 292(1), 125-129 (2001-04-26)
Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature
Sylvia Kristyanto et al.
Journal of microbiology (Seoul, Korea), 56(5), 317-323 (2018-05-04)
A novel, aerobic, Gram-stain-negative, non-motile, non-spore forming, rod-shaped bacterium, designated strain Dol 15-39T, was isolated from a seawater sample near Geoje Island in the South Sea, Republic of Korea. The strain was found to be oxidase-negative and catalase-positive. The isolate

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