17975
Ninhydrin reagent according to Stahl
for TLC derivatization
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mL | Available to ship TODAYfromMILWAUKEE | $106.00 |
About This Item
NACRES:
NA.22
UNSPSC Code:
41116105
$106.00
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Quality Level
form
liquid
technique(s)
thin layer chromatography (TLC): suitable
impurities
1-butanol, acetic acid
density
0.823 g/mL at 20 °C
General description
Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product.[1] Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.[2]
Application
1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid
Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.[2]
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) thin layer chromatography (TLC): suitable | technique(s) titration: suitable | technique(s) - | technique(s) - |
| impurities 1-butanol, acetic acid | impurities - | impurities - | impurities - |
| form liquid | form powder | form solid | form powder |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| density 0.823 g/mL at 20 °C | density - | density - | density - |
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
wgk
WGK 2
flash_point_f
93.2 °F - closed cup
flash_point_c
34 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Thin Layer Chromatography in Phytochemistry.
Hajnos WM
Science, 312-312 (2008)
B Starcher
Analytical biochemistry, 292(1), 125-129 (2001-04-26)
Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature
M K Gaitonde
The Biochemical journal, 104(2), 627-633 (1967-08-01)
1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 649007-1G | 04061826073698 |
| 17975-100ML | 04061838754431 |