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Oxidase Reagent according to Gordon-McLeod

suitable for microbiology

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Synonym(s):
Gordon-McLeod Reagent
NACRES:
NA.85

agency

according to ISO 9308-1:2014

Quality Level

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

N,N-dimethyl-p-phenylenediamine hydrochloride, 0.15 g
water, 10 mL

packaging

pkg of 100 mL

storage condition

(Tightly closed)

technique(s)

microbe id | specific enzyme detection: suitable

antibiotic activity spectrum

Gram-negative bacteria

application(s)

clinical testing
environmental
food and beverages
pharmaceutical

microbiology

suitability

Plesiomonas spp.
Aeromonas spp.
Neisseria spp.
Pseudomonas spp.
bacteria

General description

Oxidase Reagent, according to Gordon-McLeod, is a commonly used laboratory reagent in microbiology to detect bacteria that produce cytochrome c oxidase. The reagent is designed to identify this enzyme′s presence, which plays a critical role in the electron transport chain and indicates respiratory metabolism in bacteria. The Gordon-McLeod reagent contains N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride, as the active component in the reagent. When an organism produces cytochrome c oxidase, the reagent undergoes a color change reaction, turning from colorless to blue or purple. This color change is a positive result, indicating the presence of cytochrome c oxidase activity in the tested organism.

Application

Oxidase Reagent, according to Gordon-McLeod, is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce cytochrome c oxidase. The Gaby-Hadley A reagent provides a simple and efficient way to identify oxidase-positive bacteria, such as Pseudomonas, Vibrio, and some Neisseria species, while producing negative results for organisms that lack cytochrome c oxidase activity. in the food, beverage, and pharmaceutical industries.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves


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