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MilliporeSigma

18597

Triethylammonium bicarbonate buffer

1 M, suitable for HPLC, LiChropur

Synonym(s):

Triethylammonium hydrogen carbonate buffer

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100 ML

$140.00

500 ML

$478.00

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About This Item

CAS Number:
Beilstein/REAXYS Number:
3624751
MDL number:
UNSPSC Code:
12161702
NACRES:
NA.21

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form

liquid

Quality Level

quality

LiChropur

concentration

1 M

technique(s)

HPLC: suitable

impurities

≤0.05% non-volatile matter

pH

8.4-8.6 (20 °C in neat)

density

1.015-1.024

UV absorption

λ: 260 nm Amax: ≤0.04
λ: 270 nm Amax: ≤0.04
λ: 280 nm Amax: ≤0.03
λ: 290 nm Amax: ≤0.02
λ: 500 nm Amax: ≤0.02

storage temp.

2-8°C

SMILES string

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI key

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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1 of 4

This Item
T7408S245490360
technique(s)

HPLC: suitable

technique(s)

-

technique(s)

protein expression: suitable

technique(s)

-

concentration

1 M

concentration

1.0 M

concentration

2 M

concentration

~1.0 M in H2O

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

solution

form

liquid

pH

8.4-8.6 (20 °C in neat)

pH

8.5±0.1

pH

-

pH

8.4-8.6 (25 °C)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

General description

Triethylammonium bicarbonate buffer is a commonly used chromatographic eluent, volatile buffer, mobile phase and ion-pairing agent for the purification of nucleic acids and biomolecules.[1]

Application

Buffer for use in ion-exchange chromatography and electrophoresis.
Triethylammonium bicarbonate (TEAB) buffer may be used for studying proteins or noncovalent protein macromolecular complexes under “nondenaturing” conditions by electrospray ionization.[2]

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Improved preparation of 2 M triethylammonium bicarbonate
Carlson M, et al.
Green Chemistry Letters and Reviews, 8(3-4), 37-39 (2015)
D Lemaire et al.
Analytical chemistry, 73(8), 1699-1706 (2001-05-08)
The use of triethylammonium bicarbonate (TEAB) solution in electrospray mass spectrometry proved to be a very efficient way for studying proteins or noncovalent protein complexes under "nondenaturing" conditions. The low charge states observed in the mass spectra improve the separation
Peng Zhao et al.
Autophagy, 16(12), 2180-2192 (2020-01-28)
In plants, macroautophagy/autophagy has mainly been associated with stress-related processes but how it impacts normal physiological and developmental processes remains largely unexplored. Pollen germination is the critical first step toward fertilization in flowering plants. It is metabolically demanding and relies

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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