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198099

Sigma-Aldrich

Methyl violet 2B

certified by the Biological Stain Commission

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Synonym(s):
Basic Violet 1
CAS Number:
Colour Index Number:
42535
Beilstein:
8464808
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

crystalline powder

composition

Dye content, 75%

mp

137 °C (dec.) (lit.)

λmax

584 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.C\N=C1/C=C\C(C=C1)=C(/c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C24H27N3.ClH/c1-25-21-12-6-18(7-13-21)24(19-8-14-22(15-9-19)26(2)3)20-10-16-23(17-11-20)27(4)5;/h6-17H,1-5H3;1H

InChI key

JFTBTTPUYRGXDG-UHFFFAOYSA-N

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This Item
69710C0775C6158
Methyl violet 2B certified by the Biological Stain Commission

Sigma-Aldrich

198099

Methyl violet 2B

Methyl violet for microscopy (Bact., Bot., Hist.), indicator (pH 0.1-2.0), mixture of polymethylated pararosaniline hydrochlorides

Sigma-Aldrich

69710

Methyl violet

Crystal Violet certified by the Biological Stain Commission

Sigma-Aldrich

C0775

Crystal Violet

Crystal Violet ACS reagent, ≥90.0% anhydrous basis

Sigma-Aldrich

C6158

Crystal Violet

form

crystalline powder

form

powder or crystals

form

powder

form

powder

composition

Dye content, 75%

composition

-

composition

Dye content, ≥90%

composition

-

mp

137 °C (dec.) (lit.)

mp

137 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

λmax

584 nm

λmax

-

λmax

-

λmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Methyl Violet 2B is a mixture of polymethylated (4,5 and 6) pararosaniline chlorides

Application

Methyl violet 2B has been used to stain human lung fibroblast cells (A549) in in vitro antiviral assay.

Biochem/physiol Actions

Methyl violet 2B is a cationic dye with a triphenylmethane structure. It acts as a selective dye and facilitates the differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms. Methyl violet 2B is harmful if inhaled, swallowed or absorbed through skin, contact may cause damage to skin and eyes. Hence, the dye removal is of great importance.

Suitability

Certified for use in Flemming triple stain with iodine for chromosomes; Newton′s crystal violet-iodine technique for chromatin and nucleoli; Lieb′s amyloid stain.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Crystal Violet ACS reagent, ≥90.0% anhydrous basis

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C6158

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Annabelle Herrington-Symes et al.
Journal of drug targeting, 25(9-10), 881-890 (2017-08-19)
Increasingly complex proteins can be made by a recombinant chemical approach where proteins that can be made easily can be combined by site-specific chemical conjugation to form multifunctional or more active protein therapeutics. Protein dimers may display increased avidity for
Equilibrium and kinetic studies of methyl violet sorption by agricultural waste
Hameed B H
Journal of Hazardous Materials, 154(1-3), 204-212 (2008)
Methyl Violet: a Selective Agent for Differentiation of
Klebsiella pneumoniae from Enterobacter aerogenes and Other
Gram-Negative Organisms
CAMPBELL L M and ROTH I L
Applied Microbiology, 30, 258-261 (1975)
Expression of soluble and active interferon
consensus in SUMO fusion expression system
in E.coli
Peciak K
Protein Expression and Purification, 99 (2014), 18- 26 (2014)
Removal of cationic dye methyl violet 2B from water by cation exchange membranes
ShiouWu J
Journal of Membrane Science, 309(1-2), 239-245 (2008)

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