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198161

Sigma-Aldrich

Toluidine Blue O

certified by the Biological Stain Commission

Synonym(s):
Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52040
EC Number:
MDL number:
PubChem Substance ID:

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, 80%

λmax

626 nm

ε (extinction coefficient)

≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

InChI key

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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This Item
415049M9015T3260
Toluidine Blue O certified by the Biological Stain Commission

Sigma-Aldrich

198161

Toluidine Blue O

Toluidine Blue O Technical grade

Sigma-Aldrich

T3260

Toluidine Blue O

grade

certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

grade

-

form

powder

form

powder, crystals or granules

form

powder or crystals

form

powder

composition

Dye content, 80%

composition

Dye content, 65%

composition

Dye content, ≥90%

composition

Dye content, ~80%

λmax

626 nm

λmax

605 nm

λmax

-

λmax

-

ε (extinction coefficient)

≥13000 at 241-247 nm in H2O, ≥28000 at 287-293 nm in H2O, ≥40000 at 625-635 nm in H2O

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

General description

A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Biochem/physiol Actions

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Suitability

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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