MilliporeSigma
All Photos(2)

Documents

199664

Sigma-Aldrich

Sudan Black B

certified by the Biological Stain Commission

Sign Into View Organizational & Contract Pricing

Synonym(s):
Ceres black BN, Fat Black HB, Solvent Black 3
Empirical Formula (Hill Notation):
C29H24N6
CAS Number:
Molecular Weight:
456.54
Colour Index Number:
26150
Beilstein:
723248
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

mp

120-124 °C (lit.)

λmax

415 nm (2nd)
598 nm

ε (extinction coefficient)

≥20000 at 596-603 nm in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46

InChI

1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+

InChI key

YCUVUDODLRLVIC-VPHDGDOJSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
211842S4261S4136
Sudan Black B certified by the Biological Stain Commission

Sigma-Aldrich

199664

Sudan Black B

Brilliant Black BN Dye content 60 %

Sigma-Aldrich

211842

Brilliant Black BN

Sudan IV certified by the Biological Stain Commission, BioXtra

Sigma-Aldrich

S4261

Sudan IV

Sudan III BioXtra, certified by the Biological Stain Commission

Sigma-Aldrich

S4136

Sudan III

form

powder

form

powder

form

powder

form

powder

mp

120-124 °C (lit.)

mp

-

mp

199 °C (dec.) (lit.)

mp

199 °C (dec.) (lit.)

λmax

415 nm (2nd), 598 nm

λmax

407 nm (2nd), 570 nm

λmax

356-360 nm (2nd), 519-523 nm (1st)

λmax

-

ε (extinction coefficient)

≥20000 at 596-603 nm in ethanol

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

Application

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Biochem/physiol Actions

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Suitability

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

Sudan Red 7B

Sigma-Aldrich

201618

Sudan Red 7B

Sudan II Dye content 90 %

Sigma-Aldrich

199656

Sudan II

Nile Blue A Dye content ≥75 %

Sigma-Aldrich

N5632

Nile Blue A

Nile Blue A certified by the Biological Stain Commission

Sigma-Aldrich

N0766

Nile Blue A

H N Subramaniam et al.
Journal of biochemical and biophysical methods, 21(1), 9-16 (1990-06-01)
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography.
Acetylated Sudan Black B as a More Specific Histochemical Reagent for Lipides
CASSELMAN WGB
Journal of Cell Science, 321- 322 (1954)
Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals
Kupferschmidt DA
Frontiers in Neuroanatomy (2015)
John P. Greer
Wintrobe's Clinical Hematology, 1 (2009)
Effects of feeding different lipid sources on hepatic histopathology features and growth traits of broiler chickens
Tufarelli V, et.al.
Acta Histochemica, 117, 780-783 (2015)

Articles

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service