199664

Sigma-Aldrich

Sudan Black B

certified by the Biological Stain Commission

Synonym(s):
Solvent Black 3, Ceres black BN, Fat Black HB
Empirical Formula (Hill Notation):
C29H24N6
CAS Number:
Molecular Weight:
456.54
Colour Index Number:
26150
Beilstein/REAXYS Number:
723248
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

application(s)

hematology: suitable
histology: suitable

mp

120-124 °C (lit.)

λmax

415 nm (2nd)
598 nm

ε (extinction coefficient)

≥20000 at 596-603 nm in ethanol

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46

InChI

1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+

InChI key

YCUVUDODLRLVIC-VPHDGDOJSA-N

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Application

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Packaging

25 g in glass bottle

Biochem/physiol Actions

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Suitability

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

hazcat

Muta. 2

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Effects of feeding different lipid sources on hepatic histopathology features and growth traits of broiler chickens
Tufarelli V, et.al.
Acta Histochemica, 117, 780-783 (2015)
John P. Greer
Wintrobe's Clinical Hematology, 1 (2009)
H N Subramaniam et al.
Journal of biochemical and biophysical methods, 21(1), 9-16 (1990-06-01)
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography....
Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals
Kupferschmidt DA
Frontiers in Neuroanatomy (2015)
Acetylated Sudan Black B as a More Specific Histochemical Reagent for Lipides
CASSELMAN WGB
Journal of Cell Science, 321- 322 (1954)

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