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216313

Sigma-Aldrich

Methylene Violet (Bernthsen)

certified by the Biological Stain Commission, Dye content ≥65 %

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Empirical Formula (Hill Notation):
C14H12N2OS
CAS Number:
Molecular Weight:
256.32
Colour Index Number:
52041
EC Number:
MDL number:
PubChem Substance ID:

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥65%

mp

216 °C (dec.) (lit.)

solubility

soluble, clear

λmax

580 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc2N=C3C=CC(=O)C=C3Sc2c1

InChI

1S/C14H12N2OS/c1-16(2)9-3-5-11-13(7-9)18-14-8-10(17)4-6-12(14)15-11/h3-8H,1-2H3

InChI key

ALJHHTHBYJROOG-UHFFFAOYSA-N

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This Item
M9140C0775861340
Methylene Violet (Bernthsen) certified by the Biological Stain Commission, Dye content ≥65 %

216313

Methylene Violet (Bernthsen)

Methylene blue certified by the Biological Stain Commission

M9140

Methylene blue

Crystal Violet certified by the Biological Stain Commission

C0775

Crystal Violet

Thionin acetate salt certified by the Biological Stain Commission, Dye content ≥85 %

861340

Thionin acetate salt

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

powder

solubility

soluble, clear

solubility

H2O: 4mg/4ml

solubility

H2O: 1 mg/mL

solubility

-

composition

Dye content, ≥65%

composition

Dye content, ≥82%

composition

Dye content, ≥90%

composition

Dye content, ≥85%

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

200

Suitability

Certified for use as a constituent of MacNeal′s tetrachrome stain for blood.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jürgen Burhenne et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 863(2), 273-282 (2008-02-09)
Selective and sensitive methods for the determination of the cationic dye and anti-malarial methylene blue in human liquid whole blood, dried whole blood (paper spot), and plasma depending on protein precipitation and cation exchange chromatography coupled to electrospray ionisation (ESI)
Kevin E Washburn et al.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 68(2), 105-111 (2004-06-11)
The objectives of this study were to determine the optimal concentration of phenothiazine dye required to inactivate bovine viral diarrhea virus (BVDV) in goat colostrum following 60 min of illumination and determine if immunoglobulin concentration is affected by this technique.
M A Houghtaling et al.
Photochemistry and photobiology, 71(1), 20-28 (2000-01-29)
O-Methyl methylene violet (OMeMV), O-methyl bromomethylene violet (OMeBrMV) and O-methyl iodomethylene violet (OMeIMV) have been prepared in order to test their potential utility as anti-viral and anti-tumor phototoxic dyes. Rates of photosensitized toxicity of KB cells with 633 nm irradiation
H Morrison et al.
Photochemistry and photobiology, 66(2), 245-252 (1997-08-01)
The interaction of methylene violet (MV) and 4-bromomethylene violet (BrMV) with calf thymus and supercoiled phi X174 phage RF I DNA is reported. Measurements employing UV-visible absorption spectroscopy and equilibrium dialysis give evidence for the formation of complexes by each
A Skripchenko et al.
Photochemistry and photobiology, 65(3), 451-455 (1997-03-01)
Previous studies with methylene blue (MB) in red cell suspensions have demonstrated that extracellular, but not intracellular, virus can be readily photoinactivated. To test if the resistance of intracellular virus to inactivation is related to the permanent positive charge of

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