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Tryptic Soy Agar - Dehydrated Culture Media

NutriSelect® Plus, powder, suitable for microbiology

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Synonym(s):
CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soy Agar, CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soya Agar
NACRES:
NA.74

agency

according to GB 4789.40-2016
according to ISO 11731:2017
according to ISO 22964:2017

Quality Level

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
casein peptone (pancreatic), 15 g/L
sodium chloride, 5 g/L
soya peptone (papainic), 5 g/L

packaging

bottle of 2.5 kg
bottle of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

color

light yellow

final pH

7.3±0.2 (25 °C)

application(s)

agriculture
bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
surface monitoring
veterinary

microbiology

suitability

Enterococcus spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Bacillus spp.
nonselective for Bacteroides spp.
nonselective for Campylobacter spp.
nonselective for Candida spp.
nonselective for Cronobacter spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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General description

Tryptic Soy Agar is a general-purpose culture medium for the isolation and cultivation of fastidious and non-fastidious microorganisms or the maintenance of stock culture. It is a complex medium for the cultivation and isolation of a wide range of bacteria, yeasts, and molds. The formulation of the TSA medium contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids, and longer-chained peptides. Sodium chloride maintains the osmotic equilibrium, and the natural sugars from soy peptone are the energy source.

Application

Tryptic Soy Agar is used for the pre-cultivation and enumeration (E. coli) by membrane filtration technique. It is suitable for the cultivation of both aerobic and anaerobic bacteria. Since it does not contain the X and V factors, it is suitable for the identification of Haemophilus sp. by adding X (Hemin) and V (DPN=NAD) factors strips.

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 40 g of dehydrated media in 1 litre of purified filtered water. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Cronobacter spp. are frequently found on vegetables, meat, fermented bread, dairy and in baby food. Consumption of contaminated powdered infant formula milk can result in sepsis, infant meningitis and necrotizing enterocolitis.

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

Specific agars and broths support detection and cultivation of Campylobacter, requiring complex media with essential supplements.

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.

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