Autoclaving affects the pH of the dissolved medium, hence it is preferable to check the pH after autoclaving. pH should be checked at a temperature between 20-25°C. Adjustments can be made with HCl/NaOH if needed, while stirring.
22091
Tryptic Soy Agar - Dehydrated Culture Media
Tryptic Soy Agar - Dehydrated Culture Media
NutriSelect® Plus, powder, suitable for microbiology
Synonym(s):
CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soy Agar, CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soya Agar
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500 G
$110.00
2.5 KG
$485.00
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500 G
$110.00
2.5 KG
$485.00
About This Item
UNSPSC Code:
41171605
NACRES:
NA.74
Recommended Products
agency
according to GB 4789.40-2016
according to ISO 11731:2017
according to ISO 22964:2017
Quality Level
sterility
non-sterile
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 15 g/L
casein peptone (pancreatic), 15 g/L
sodium chloride, 5 g/L
soya peptone (papainic), 5 g/L
packaging
bottle of 2.5 kg
bottle of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | specific enzyme detection: suitable
microbiological culture: suitable
color
light yellow
final pH
7.3±0.2 (25 °C)
application(s)
agriculture
bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
surface monitoring
veterinary
microbiology
suitability
Enterococcus spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Bacillus spp.
nonselective for Bacteroides spp.
nonselective for Campylobacter spp.
nonselective for Candida spp.
nonselective for Cronobacter spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms
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General description
Tryptic Soy Agar is a general-purpose culture medium for the isolation and cultivation of fastidious and non-fastidious microorganisms or the maintenance of stock culture. It is a complex medium for the cultivation and isolation of a wide range of bacteria, yeasts, and molds. The formulation of the TSA medium contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids, and longer-chained peptides. Sodium chloride maintains the osmotic equilibrium, and the natural sugars from soy peptone are the energy source.
Application
Tryptic Soy Agar is used for the pre-cultivation and enumeration (E. coli) by membrane filtration technique. It is suitable for the cultivation of both aerobic and anaerobic bacteria. Since it does not contain the X and V factors, it is suitable for the identification of Haemophilus sp. by adding X (Hemin) and V (DPN=NAD) factors strips.
Features and Benefits
NutriSelect® Plus are powdered culture media offering
- Quality control with growth promotion test according to industry standards
- Quality assurance acc. to ISO 9001
- Cost-efficient powdered media
Preparation Note
Suspend 40 g of dehydrated media in 1 litre of purified filtered water. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hi everyone and good afternoon My question is about checking of PH of Media, in which conditions we should check PH of the medium before autoclaving or after autoclaving
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