Tryptic Soy Broth

suitable for microbiology, NutriSelect® Plus

Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB, Tryptone Soya Broth
MDL number:

Quality Level


according to ISO 10273:2017
according to ISO 21871:2006





shelf life

limited shelf life, expiry date on the label


casein peptone (pancreatic), 17 g/L
dipotassium hydrogen phosphate, 2.5 g/L
glucose, 2.5 g/L
sodium chloride, 5 g/L
soya peptone (papain digest.), 3 g/L


NutriSelect® Plus


bioburden testing: suitable
microbiological culture: suitable
microbiology: suitable
pathogen testing: suitable
sample preparation: suitable
water monitoring: suitable

final pH

7.3±0.2 (25 °C)

Featured Industry

Cleaning Products
Clinical Testing
Food and Beverages
Personal Care


nonselective for Bacillus spp.
nonselective for Bacteroides spp.
nonselective for Campylobacter spp.
nonselective for Candida spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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Related Categories


For general purpose media for bacteria and for confirmation of Campylobacter jejuni by means of the motility test.

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Other Notes

Capability of selective media to detect heat-injured Shigella flexneri

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Lucia Hrncirova et al.
Folia microbiologica, 64(4), 497-508 (2019-01-19)
The aim of this work was to test the hypothesis that antimicrobial food additives may alter the composition of human gut microbiota by selectively suppressing the growth of susceptible gut microbes. To explore the influence of antimicrobial food additives on...
S K Urwyler et al.
Letters in applied microbiology, 62(2), 130-137 (2015-11-20)
Fast microbial identification is becoming increasingly necessary in industry to improve microbial control and reduce biocide consumption. We compared the performances of two systems based on MALDI-TOF MS (VITEK MS and BIOTYPER) and two based on biochemical testing (BIOLOG, VITEK...
J.L. Smith et al.
Journal of Food Protection, 53, 141-141 (1990)
H S Nagendra Prasad et al.
Microbial pathogenesis, 127, 106-115 (2018-12-07)
The synthesized potent piperazine analog ChDiPiCa was characterised by various spectroscopic techniques and for the first time evaluated functional membrane microdomain (FMM) disassembly in methicillin-resistant Staphylococcus aureus (MRSA). The ChDiPiCa showed excellent in vitro biocidal activity against MRSA at 26 μg/mL...
Omar F Prado-Rebolledo et al.
Avian pathology : journal of the W.V.P.A, 46(1), 90-94 (2016-08-23)
Two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (FloraMax-B11
On the Trail of Campylobacter - The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis. However, other species, like C. fetus, which causes spontaneous abortions, have also been associated with human illness. Campylobacter are Gram-negative, spiral-shaped, microaerophilic and motile bacteria with uni- or bi-polar fl agella
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Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.
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Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
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