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221198

Formamide

ACS reagent, ≥99.5%

Synonym(s):

Amide C1, Formic amide

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About This Item

Linear Formula:
HCONH2
CAS Number:
75-12-7
Molecular Weight:
45.04
EC Number:
200-842-0
UNSPSC Code:
12352111
PubChem Substance ID:
24853220
Beilstein/REAXYS Number:
505995
MDL number:
MFCD00007941
Assay:
≥99.5%
Grade:
ACS reagent
Technique(s):
PCR: suitable
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
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grade

ACS reagent

Quality Segment

200

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

assay

≥99.5%

form

liquid

autoignition temp.

932 °F

technique(s)

PCR: suitable

color

APHA: ≤10

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

transition temp

freezing point 2.0-3.0 °C

density

1.134 g/mL at 25 °C (lit.)

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

General description

Our premium ACS solvents are ideal for routine chemical synthesis, drying, purification, and critical labware cleaning. They meet or exceed the rigorous standards of the American Chemical Society (ACS), ensuring high-quality results for your research needs.

Premium ACS Solvents: Our solvents meet or exceed the stringent standards set by the American Chemical Society, ensuring high quality and reliability for your laboratory applications.

Replicable and Publishable Results: Designed for consistency, our solvents deliver results that can be reliably reproduced, making them ideal for research that requires publication.

Versatile Applications: Suitable for routine chemical synthesis, drying, purification, and critical labware cleaning, our solvents cater to a wide range of research needs in the laboratory.

Application

The addition of formamide may enhance the specificity and efficiency of amplification of PCR (polymerase chain reaction). It may be used as an ammonia equivalent for the palladium-catalyzed aminocarbonylation of 1-bromo-3-trifluoromethylbenzene to form 3-(trifluoromethyl)benzamide.
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

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1.09684F90374610-OP
Formamide ACS reagent, ≥99.5%

Sigma-Aldrich

221198

Formamide

Formamide for analysis EMSURE®

Supelco

1.09684

Formamide

Formamide ≥99.5% (GC), BioReagent, Molecular Biology

Sigma-Aldrich

F9037

Formamide

Formamide OmniPur® Grade, >= 99%, Deionized

Millipore

4610-OP

Formamide

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

100

assay

≥99.5%

assay

≥99.2% (N)

assay

≥99.5% (GC)

assay

≥99% (GC)

grade

ACS reagent

grade

reagent grade

grade

Molecular Biology

grade

-

technique(s)

PCR: suitable

technique(s)

-

technique(s)

electrophoresis: suitable, immunohistochemistry: suitable

technique(s)

hybridization: suitable

form

liquid

form

liquid

form

liquid

form

viscous liquid (slightly)

refractive index

n20/D 1.447 (lit.)

refractive index

n20/D 1.447 (lit.)

refractive index

n20/D 1.447 (lit.)

refractive index

n20/D 1.447 (lit.)

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pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

305.6 °F

flash_point_c

152 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Questions

  1. What is the best media for diluting this solution?

    1 answer

    1. This material is miscible with water, methanol, ethanol, acetone, acetic acid, dioxane, ethylene glycol, glycerol, and phenol. This product has not been deionized, which is required for use in molecular biology applications. For use in nucleic acid hybridization and in gel loading solution, the following protocol is suggested for deionization:

      1. Mix 1 gram of a mixed bed resin (such as Sigma Prod. No. M8032) with 10 mL of formamide.
      2. Stir for approximately 1 hour.
      3. Filter two times through Whatman #1 filter.
      4. Sterile filter and store for a single use at -20 C; stable up to 6 months.

      If the deionized formamide is stored at room temperature, deionize every 2 weeks. The pH of a 0.5 M solution is approximately 5.0 before deionization and approximately 6.8 after deionization. Formamide with a yellowish appearance requires deionization.

      Note that this product has not been tested for suitability in molecular biology. Please see the links below to review Formamide product suitable for Molecular Biology:
      https://www.sigmaaldrich.com/product/sigma/47671
      https://www.sigmaaldrich.com/product/mm/344206m

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