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222550

Sigma-Aldrich

Nile Blue chloride

Dye content 85 %

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Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
Molecular Weight:
353.85
Colour Index Number:
51180
Beilstein/REAXYS Number:
3923952
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 85%

solubility

water: 1 mg/mL, opaque, blue to very deep blue

λmax

638 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34

InChI

1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H

InChI key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

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1 of 4

This Item
A3157B5388195243
vibrant-m

222550

Nile Blue chloride

vibrant-m

A3157

Alcian Blue 8GX

vibrant-m

B5388

Brilliant Cresyl Blue ALD

vibrant-m

195243

Naphthol Blue Black

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

λmax

638 nm

λmax

-

λmax

-

λmax

618 nm

solubility

water: 1 mg/mL, opaque, blue to very deep blue

solubility

water: 1 mg/mL, blue

solubility

water: 1 mg/mL, blue to very deep blue

solubility

water: 1 mg/mL, opaque, blue to very deep blue

composition

Dye content, 85%

composition

Dye content, ≥50%

composition

Dye content, ≥60%

composition

Dye content, 80%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Biochem/physiol Actions

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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