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229253

Sigma-Aldrich

Carminic acid

Synonym(s):
Natural Red 4
Empirical Formula (Hill Notation):
C22H20O13
CAS Number:
Molecular Weight:
492.39
Colour Index Number:
75470
Beilstein:
71651
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

assay

≥90% (HPLC)

Quality Level

form

powder

optical activity

[α]20/D +3.1°, c = 1 in H2O

mp

136 °C

λmax

495 nm

ε (extinction coefficient)

≥13000 at 222-228  nm in ethanol at 0.03 g/L
≥18000 at 275-281 nm in ethanol at 0.03 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1c(C(O)=O)c(O)cc2C(=O)c3c(O)c(O)c([C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)c(O)c3C(=O)c12

InChI

1S/C22H20O13/c1-4-8-5(2-6(24)9(4)22(33)34)13(25)10-11(15(8)27)16(28)12(18(30)17(10)29)21-20(32)19(31)14(26)7(3-23)35-21/h2,7,14,19-21,23-24,26,28-32H,3H2,1H3,(H,33,34)/t7-,14-,19+,20-,21+/m1/s1

InChI key

DGQLVPJVXFOQEV-JNVSTXMASA-N

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General description

Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilized in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.

Application

Carminic acid (CI 75470) is used in Orth′s lithium carmine technique for nuclear staining, Best′s stain for glycogen, and Southgates′s mucicarmine method.

Packaging

1, 5 g in glass bottle

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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