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244813

Sigma-Aldrich

Reactive Blue 4

Dye content 35 %

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Synonym(s):
1-Amino-4-[3-(4,6-dichlorotriazin-2-ylamino)-4-sulfophenylamino]anthraquinone-2-sulfonic acid, Procion® blue MX-R
Empirical Formula (Hill Notation):
C23H14Cl2N6O8S2
CAS Number:
Molecular Weight:
637.43
Colour Index Number:
61205
Beilstein:
602258
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 35%

λmax

595 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Nc1c(cc(Nc2ccc(c(Nc3nc(Cl)nc(Cl)n3)c2)S(O)(=O)=O)c4C(=O)c5ccccc5C(=O)c14)S(O)(=O)=O

InChI

1S/C23H14Cl2N6O8S2/c24-21-29-22(25)31-23(30-21)28-12-7-9(5-6-14(12)40(34,35)36)27-13-8-15(41(37,38)39)18(26)17-16(13)19(32)10-3-1-2-4-11(10)20(17)33/h1-8,27H,26H2,(H,34,35,36)(H,37,38,39)(H,28,29,30,31)

Inchi Key

RTLULCVBFCRQKI-UHFFFAOYSA-N

Gene Information

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1 of 4

This Item
R8001B0149302643
Reactive Blue 4 Dye content 35 %

Sigma-Aldrich

244813

Reactive Blue 4

Brilliant Blue R Dye content ~50 %, Technical grade

Sigma-Aldrich

B0149

Brilliant Blue R

Trypan Blue Dye content 60 %

Sigma-Aldrich

302643

Trypan Blue

composition

Dye content, 35%

composition

Dye content, ~50%

composition

Dye content, ~50%

composition

Dye content, 60%

λmax

595 nm

λmax

-

λmax

-

λmax

607 nm

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Reactive blue 4 (C.l. 61205) is a dichlorotriazine dye also known as procion brilliant blue MX-R, procion brilliant blue M.

Application

Reactive blue dyes have been used for labeling antibodies. Such anthraquinone dyes are widely used as immobilized ligands in systems for protein separations. Reactive blue 4 is used as a tracer to assess apical leakage in dental fillings. It is routinely used to stain proteins on electrophoretic gels.

Legal Information

Procion is a registered trademark of Dystar Textilfarben GmbH & Co.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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K Vijayaraghavan et al.
Journal of hazardous materials, 153(1-2), 478-486 (2007-10-05)
Competition of Reactive red 4 (RR4), Reactive orange 16 (RO16) and Basic blue 3 (BB3) during biosorption of Reactive blue 4 (RB4) by polysulfone-immobilized protonated Corynebacterium glutamicum (PIPC) was investigated in batch and column mode of operations. Through potentiometric titrations
S A Medina-Moreno et al.
Bioresource technology, 123, 452-462 (2012-09-04)
A biodecolorization model that considers the simultaneous mechanism of biosorption and biodegradation of a synthetic dye by immobilized white-rot fungus Trametes subectypus B32 in a fixed bed bioreactor was developed. The model parameters (biokinetic, biosorption and macroscopic transport) were determined
Arthur Raj Binupriya et al.
Colloids and surfaces. B, Biointerfaces, 76(1), 179-185 (2009-11-27)
Bacillus subtilis a gram positive bacteria and its extracellular polysaccharide were used in free form as well as immobilized form as biosorbent for sequestration of an anionic dye, Reactive Blue 4 (RB) in aqueous phase. The dye uptake enhanced with
Wei-Feng Chang et al.
Analytical biochemistry, 411(2), 236-240 (2010-12-29)
An improved dye immunochromatographic test (DICT) using polylysine (PL) as conjugate spacer loading dye molecules to enhance chromophor color intensity with the potential of a simultaneous multicolored assay has been developed. To construct this new effective chromophor, a dyeing process
Feiyan Wu et al.
Journal of chromatography. A, 1134(1-2), 45-50 (2006-10-13)
Monodisperse porous particles, poly(glycidylmethacrylate-co-ethylenedimethacrylate), P(GMA-EDMA) beads with diameter of 7 microm were prepared by a single-step swelling and polymerization method. The polymer particles were advantaged through immobilization of Procion Bule MX-R, which was incubated by epichlorohydrin via the epoxide groups

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