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25668

Sigma-Aldrich

Chloroform – isoamyl alcohol mixture

BioUltra, Molecular Biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Synonym(s):

Chloroform:Isoamyl Alcohol Solvent Mix

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100 ML
$113.00
500 ML
$387.60

About This Item

MDL number:
UNSPSC Code:
12352401
PubChem Substance ID:
NACRES:
NA.25
grade:
Molecular Biology
assay:
≥99.5% (chloroform + isoamyl alcohol, GC)
technique(s):
GC/GC: suitable

$113.00


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Request a Bulk Order

grade

Molecular Biology

Quality Level

product line

BioUltra

assay

≥99.5% (chloroform + isoamyl alcohol, GC)

form

liquid

technique(s)

GC/GC: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% free alkali (as NH3)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤0.5 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.1
λ: 280 nm Amax: 0.07

SMILES string

ClC(Cl)Cl.CC(C)CCO

InChI

1S/C5H12O.CHCl3/c1-5(2)3-4-6;2-1(3)4/h5-6H,3-4H2,1-2H3;1H

InChI key

BKHZIBWEHPHYAI-UHFFFAOYSA-N

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This Item
256667761777619
Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

assay

≥99.5% (chloroform + isoamyl alcohol, GC)

assay

≥99.5% (chloroform + isoamyl alcohol, GC)

assay

-

assay

-

form

liquid

form

liquid

form

liquid

form

liquid

UV absorption

λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.07

UV absorption

λ: 260 nm Amax: 0.15, λ: 280 nm Amax: 0.1

UV absorption

-

UV absorption

λ: 330 nm Amax: 0.2, λ: 405 nm Amax: 0.1, λ: 510 nm Amax: 0.1

cation traces

Al: ≤0.5 mg/kg, Bi: ≤0.1 mg/kg, Cd: ≤0.05 mg/kg, Cr: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, Li: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Na: ≤0.5 mg/kg, Pb: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg, Ba: ≤0.1 mg/kg, Co: ≤0.02 mg/kg, K: ≤0.5 mg/kg, Mo: ≤0.1 mg/kg, Sr: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Mg: ≤0.1 mg/kg, Cu: ≤0.02 mg/kg, Ni: ≤0.02 mg/kg

cation traces

Al: ≤0.5 mg/kg, Ba: ≤0.1 mg/kg, Bi: ≤0.1 mg/kg, Ca: ≤0.5 mg/kg, Cd: ≤0.05 mg/kg, Co: ≤0.02 mg/kg, Cr: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Fe: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Li: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Mo: ≤0.1 mg/kg, Na: ≤0.5 mg/kg, Ni: ≤0.02 mg/kg, Pb: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg

cation traces

-

cation traces

-

Application

Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.

Components

mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v)

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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