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26049

Supelco

Chlorophosphonazo III

for spectrophotometric det. of alkaline earth metals

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Synonym(s):
Bis(4-chloro-2-phosphonobenzolazo)chromotropic acid
Empirical Formula (Hill Notation):
C22H16Cl2N4O14P2S2
CAS Number:
Molecular Weight:
757.36
Beilstein:
2934871
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

form

powder

Quality Level

quality

for spectrophotometric det. of alkaline earth metals

technique(s)

UV/Vis spectroscopy: suitable

SMILES string

Oc1c(\N=N\c2ccc(Cl)cc2P(O)(O)=O)c(cc3cc(c(\N=N\c4ccc(Cl)cc4P(O)(O)=O)c(O)c13)S(O)(=O)=O)S(O)(=O)=O

InChI

1S/C22H16Cl2N4O14P2S2/c23-10-1-3-12(14(7-10)43(31,32)33)25-27-19-16(45(37,38)39)5-9-6-17(46(40,41)42)20(22(30)18(9)21(19)29)28-26-13-4-2-11(24)8-15(13)44(34,35)36/h1-8,29-30H,(H2,31,32,33)(H2,34,35,36)(H,37,38,39)(H,40,41,42)/b27-25+,28-26+

InChI key

LMBABJNSZGKTBA-NBHCHVEOSA-N

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This Item
405329S4131A1010
Chlorophosphonazo III for spectrophotometric det. of alkaline earth metals

Supelco

26049

Chlorophosphonazo III

Sudan III Technical grade

Sigma-Aldrich

S4131

Sudan III

Arsenic(III) oxide ReagentPlus®, ≥99.0%

Sigma-Aldrich

A1010

Arsenic(III) oxide

quality

for spectrophotometric det. of alkaline earth metals

quality

-

quality

-

quality

-

technique(s)

UV/Vis spectroscopy: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

Chlorophosphonazo III is a highly sensitive reagent and can be used for the spectrophotometric determination of zirconium, titanium, thorium and scandium. It also finds application as an extractant, along with 1-butanol as solvent, for determination of micromolar concentrations of Nd(III), Th(IV) and U(VI) in brine. Above all that, it has two long wavelength maxima, one at 620 nm and one at 680 nm.

Application

Chlorophosphonazo III was used as a chromogenic agent along with PVC, during the determination of calcium via a disposable optical sensor.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the spectrophotometric determination of alkaline earth metals

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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P Wohlfart et al.
Biochimica et biophysica acta, 1022(3), 283-290 (1990-03-01)
We have characterised the spectroscopic properties of the metallochromic dye dichlorophosphonazo III and describe its use for the determination of changes of Mg2+ concentration in the micromolar range. Using a previously described reconstitution procedure, we incorporated the cGMP-gated channel from
Simultaneous Spectrophotometric Determination of Calcium and Magnesium with Chlorophosphonazo III.
Ferguson JW.
Analytical Chemistry, 36 (4), 796-799 (1964)
Determination of calcium by a single-use optical sensor.
Capitan VLF
Sensors and Actuators B, Chemical, 71 (1), 140-146 (2000)
S M Baylor et al.
The Journal of physiology, 331, 179-210 (1982-10-01)
1. Absorbance changes were measured following stimulation of single muscle fibres injected with the metallochromic indicator dye Arsenazo III. Two dye-related signals can be clearly resolved: (1) an early, transient isotropic signal that appears to be due to the formation
Wei-Wen Liu et al.
Biomedical optics express, 12(2), 1154-1166 (2021-03-09)
This study demonstrates that chlorophosphonazo III (CPZ III) can be used as a contrast agent for photoacoustic calcium imaging. CPZ III can pass across the plasma membrane for labeling intracellular Ca2+ without cytotoxicity. In optical-resolution photoacoustic microscopy (OR-PAM), the photoacoustic

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