MilliporeSigma
All Photos(1)

Documents

28514

Sigma-Aldrich

Methylene Blue hydrate

≥95% (calc. to the dried substance)

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
Beilstein:
3599847
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
MA.02

Quality Level

Assay

≥95% (calc. to the dried substance)

form

powder

impurities

≤1% insoluble in ethanol

loss

≤22% loss on drying, 105 °C

solubility

ethanol: insoluble ≤1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

Inchi Key

WQVSELLRAGBDLX-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
66720M914066719
Methylene Blue hydrate ≥95% (calc. to the dried substance)

Sigma-Aldrich

28514

Methylene Blue hydrate

Methylene Blue hydrate purum p.a., for microscopy (Bact., Bot., Hist., Vit.), ≥97.0% (calc. based on dry substance, AT)

Sigma-Aldrich

66720

Methylene Blue hydrate

Methylene blue certified by the Biological Stain Commission

Sigma-Aldrich

M9140

Methylene blue

Methylene Blue hydrate meets analytical specification of BP 73

Sigma-Aldrich

66719

Methylene Blue hydrate

form

powder

form

-

form

powder

form

powder

solubility

ethanol: insoluble ≤1%

solubility

-

solubility

H2O: 4mg/4ml

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

hematology
histology
pharmaceutical (small molecule)

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Methylene blue is bis(dimethylamino)phenothiazine hydrochloride (tetramethylthionine),
C16H18N3SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.

Application

Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:
  • in the preparation of methylene blue (MB) solution
  • to stain zymograms -to label the injection site in the tissue slices
  • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Wei Guo et al.
Acta pharmacologica Sinica, 41(3), 423-431 (2019-06-15)
Indoleamine 2,3-dioxygenase 1 (IDO1) is emerging as a promising therapeutic target for the treatment of malignant tumors characterized by dysregulated tryptophan metabolism. However, the antitumor efficacy of existing small-molecule IDO1 inhibitors is still unsatisfactory, and the underlying mechanism remains largely
Paul J Derry et al.
Nanoscale, 11(22), 10791-10807 (2019-05-28)
Previously, our group reported on the promising efficacy of poly(ethylene glycol)-hydrophilic carbon clusters (PEG-HCCs) to work as broadly active and high capacity antioxidants in brain ischemia and injury models including stroke and traumatic brain injury coupled with hemorrhagic shock. PEG-HCCs

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service