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318604

Ammonium chloride-ammonium hydroxide buffer solution

in H2O

Synonym(s):

Ammonium Buffer Solution

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About This Item

NACRES:
NA.21
PubChem Substance ID:
329753929
UNSPSC Code:
12161705
MDL number:
MFCD00134124
Pricing and availability is not currently available.
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form

liquid

Quality Level

100

composition

NH4Cl, 1 wt. % , NH4OH, 4 wt. % (pH 10 to 11)

concentration

in H2O

pH

10-11

density

0.99 g/mL at 25 °C

SMILES string

N.N.O.Cl

InChI

1S/ClH.2H3N.H2O/h1H;2*1H3;1H2

InChI key

XEXFVRMLYUDDJY-UHFFFAOYSA-N

Application

Ammonium chloride-ammonium hydroxide buffer solution may be used to crystallize proteins.

Biochem/physiol Actions

Ammonium chloride is the salt component of ammonium chloride-ammonium hydroxide buffer system that completely inhibits the BVDV infection of MDBK cells when present at a concentration of 50 mM .[1] Viruses treated with ammonium chloride (NH4Cl) results in loss of infectivity because it neutralizes the pH of endosome. Hence, viruses with low pH-dependent fusion activity became incapable to fuse their envelope with endosomal membrane.[2]

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3388189035883264
Ammonium chloride-ammonium hydroxide buffer solution in H2O

Sigma-Aldrich

318604

Ammonium chloride-ammonium hydroxide buffer solution

Ammonium hydroxide solution 28% NH3 in H2O, ≥99.99% trace metals basis

Sigma-Aldrich

338818

Ammonium hydroxide solution

Triethylammonium acetate buffer volatile buffer, ~1.0 M in H2O

Sigma-Aldrich

90358

Triethylammonium acetate buffer

HEPES buffer solution 1 M in H2O

Sigma-Aldrich

83264

HEPES buffer solution

concentration

in H2O

concentration

28% NH3 in H2O

concentration

1 M, ~1.0 M in H2O

concentration

1 M in H2O, 238 g/L HEPES in H2O

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

pH

10-11

pH

11.7 (20 °C)

pH

7.0

pH

5.5±0.5

density

0.99 g/mL at 25 °C

density

0.9 g/mL at 25 °C

density

1.002 g/mL at 20 °C

density

1.07 g/mL at 20 °C

composition

NH4Cl, 1 wt. %

composition

-

composition

-

composition

-

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000479821
0000547869
0000526301
0000547869
0000526301

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Thomas Krey et al.
Journal of virology, 79(7), 4191-4200 (2005-03-16)
The route of internalization of the pestivirus bovine viral diarrhea virus (BVDV) was studied by using different chemical and biophysical inhibitors of endocytosis. Expression of the dominant-negative mutant Dyn(K44A) of the GTPase dynamin in MDBK cells, as well as the
A Takada et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(26), 14764-14769 (1998-02-07)
Ebola virus causes hemorrhagic fever in humans and nonhuman primates, resulting in mortality rates of up to 90%. Studies of this virus have been hampered by its extraordinary pathogenicity, which requires biosafety level 4 containment. To circumvent this problem, we
Julia S Brunner et al.
Nature communications, 11(1), 431-431 (2020-01-24)
Multinucleated giant cells (MGCs) are implicated in many diseases including schistosomiasis, sarcoidosis and arthritis. MGC generation is energy intensive to enforce membrane fusion and cytoplasmic expansion. Using receptor activator of nuclear factor kappa-Β ligand (RANKL) induced osteoclastogenesis to model MGC

Global Trade Item Number

SKUGTIN
318604-500ML04061826694527
318604-2L04061826694510

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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