PESTANAL®, analytical standard

Ethyl O-[5-(2-chloro-α,α,α-trifluoro-p-tolyloxy)-2-nitrobenzoyl]-DL-lactate, 2-Ethoxy-1-methyl-2-oxoethyl 5-(2-chloro-4-trifluoromethylphenoxy)-2-nitrobenzoate
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:
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analytical standard

Quality Level

product line




shelf life

limited shelf life, expiry date on the label


HPLC: suitable
gas chromatography (GC): suitable

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General description

Lactofen is a broad-spectrum post-emergent herbicide. It acts by inhibiting protoporphyrinogen oxidase by causing lipid peroxidation of the cell membrane.


Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Lactofen may be used as an analytical reference standard for the determination of the analyte in:
  • Environmental samples by high performance liquid chromatography (HPLC) coupled to tandem mass spectrometric detector (MS/MS), ultraviolet (UV) detector and circular dichroism (CD) detector.
  • Fruit and wheat flour matrices by on-line turbulent flow chromatography (TFC) combined with LC-MS/MS.
  • Agricultural products using gas chromatography coupled to flame photometric detector (GC-FPD).

Legal Information

PESTANAL is a registered trademark of Sigma-Aldrich International GmbH


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Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


UN 3077 9 / PGIII

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Irani Mukherjee et al.
Bulletin of environmental contamination and toxicology, 79(1), 49-52 (2007-06-30)
Lactofen, a member of the diphenyl ether chemical family, shows great potential for the control of broadleaf weeds associated with leguminous crops. It presents a high degree of selectivity when applied post-emergence to soybean and peanut crops. This paper presents...
Coupled turbulent flow chromatography: LC-MS/MS method for the analysis of pesticide residues in grapes, baby food and wheat flour matrices.
Hollosi L, et al.
Chromatographia, 75(23-24), 1377-1393 (2012)
Serena Landini et al.
Phytochemistry, 62(6), 865-874 (2003-02-19)
Lactofen, the active ingredient of the soybean disease resistance-inducing herbicide, Cobra, induces large accumulations of isoflavone conjugates and aglycones in soybean tissues. The predominant isoflavones induced in cotyledon tissues are daidzein (and its conjugates) and formononetin and glycitein aglycones. The...
Rosana N Malpassi
Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al, 30(1), 51-56 (2006-07-19)
Eleusine indica and Portulaca oleracea are two common weeds in peanut crops in southern Córdoba. Two chemicals are frequently used to control them, quizalofop for grasses and lactofen for dicots. The objective is to study the effects of quizalofop and...
Bo Liang et al.
Journal of agricultural and food chemistry, 58(17), 9711-9715 (2010-08-18)
The diphenyl ether herbicide lactofen is commonly used to control broadleaf weeds. Once released into the environment, this herbicide is subject to microbial reactions. This study describes the biotransformation of lactofen by Brevundimonas sp. LY-2 isolated from enrichment cultures inoculated...

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