34037

Supelco

Ochratoxin A solution

10 μg/mL in acetonitrile, analytical standard

Empirical Formula (Hill Notation):
C20H18ClNO6
CAS Number:
Molecular Weight:
403.81
Beilstein/REAXYS Number:
8169012
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.24

Quality Level

grade

analytical standard

shelf life

limited shelf life, expiry date on the label

concentration

10 μg/mL in acetonitrile

application(s)

HPLC: suitable
gas chromatography (GC): suitable

solubility

methanol: soluble 5 mg/mL, clear, yellow(lit.)

Featured Industry

Cleaning Products
Cosmetics
Food and Beverages
Personal Care

format

single component solution

storage temp.

−20°C

SMILES string

C[C@@H]1Cc2c(Cl)cc(c(O)c2C(=O)O1)C(=O)N[C@@H](Cc3ccccc3)C(O)=O

InChI

1S/C20H18ClNO6/c1-10-7-12-14(21)9-13(17(23)16(12)20(27)28-10)18(24)22-15(19(25)26)8-11-5-3-2-4-6-11/h2-6,9-10,15,23H,7-8H2,1H3,(H,22,24)(H,25,26)/t10-,15+/m1/s1

InChI key

RWQKHEORZBHNRI-BMIGLBTASA-N

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General description

Certan Vial
Ochratoxin is a mycotoxin, produced by genus Aspergillus and Penicillium. Ochratoxin producers are widely distributed in foods such as cereals. Ochratoxins are isocoumarin derivatives. A quantitative detection method for ochratoxin A by using aptamer (single-stranded oligonucleotides selected in vitro to bind to molecular targets) is reported.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Ochratoxin A (OTA) was used in quantification of OTA in table wine by immunoaffinity column clean-up and high-performance liquid chromatography. It was used as analytical standard in sample clean-up method based on immuno-ultrafiltration for the analysis of OTA in cereals. It was used as analytical standard to study the influence of OTA on the rat embryonic neural cells cultured in high density “micromass cultures”.

Pictograms

FlameExclamation mark

Signal Word

Danger

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

UN 1648 3 / PGII

WGK Germany

WGK 2

Flash Point(F)

35.6 °F - closed cup

Flash Point(C)

2 °C - closed cup

Certificate of Analysis

Certificate of Origin

Iwona Wilk-Zasadna et al.
International journal of molecular sciences, 10(1), 37-49 (2009-04-01)
Embryonic midbrain micromass cultures were exposed for five days to ochratoxin A (OTA) at seven concentrations (ranging from 0.16 to 10 microg/mL). Cell viability was assessed in neutral red uptake test (NRU), and differentiation - by immunoenzymatic determination of structural...
Jorge A Cruz-Aguado et al.
Journal of agricultural and food chemistry, 56(22), 10456-10461 (2008-11-06)
This work describes the identification of an aptamer that binds with high affinity and specificity to ochratoxin A (OTA), a mycotoxin that occurs in wheat and other foodstuffs, and a quantitative detection method for OTA based on the use of...
Elisabeth Viktoria Reiter et al.
Analytical and bioanalytical chemistry, 400(8), 2615-2622 (2011-04-05)
The paper presents a new sample clean-up method based on immuno-ultrafiltration for the analysis of ochratoxin A in cereals. In contrast to immunoaffinity chromatography, in immuno-ultrafiltration, the antibodies are used in non-immobilised form. Ochratoxin A was extracted with ACN/water (60/40...
A Visconti et al.
Journal of chromatography. A, 864(1), 89-101 (2000-01-12)
A new and accurate method to quantify ochratoxin A (OA) in table wine has been developed. The method uses commercial immunoaffinity columns for clean-up and high-performance liquid chromatography (HPLC) with fluorescence detection for quantification of the toxin. Wine was diluted...
Xia Geng et al.
Analytical and bioanalytical chemistry, 405(8), 2443-2449 (2013-01-31)
By taking advantage of the intrinsic fluorescence of ochratoxin A (OTA), we present a fluorescence anisotropy approach for rapid analysis of the interactions between OTA and aptamers. The specific binding of OTA with a 36-mer aptamer can induce increased fluorescence...

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