MilliporeSigma
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365548

Sigma-Aldrich

Direct Red 80

Dye content 25 %

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Synonym(s):
Sirius Red
Empirical Formula (Hill Notation):
C45H26N10Na6O21S6
CAS Number:
Molecular Weight:
1373.07
Colour Index Number:
35780
Beilstein:
8185575
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 25%

λmax

528 nm

ε (extinction coefficient)

≥12000 at 277-283 nm in H2O
≥15000 at 223-235 nm in H2O
≥32000 at 524-530 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c(cc4cc(NC(=O)Nc5ccc6c(O)c(\N=N\c7ccc(cc7S([O-])(=O)=O)\N=N\c8ccc(cc8)S([O-])(=O)=O)c(cc6c5)S([O-])(=O)=O)ccc14)S([O-])(=O)=O

InChI

1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6/b50-48+,51-49+,54-52+,55-53+;;;;;;

InChI key

YIQKLZYTHXTDDT-IJPLDVMMSA-H

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This Item
458848O0625N3020
Direct Red 80 Dye content 25 %

Sigma-Aldrich

365548

Direct Red 80

Allura Red AC Dye content 80 %

Sigma-Aldrich

458848

Allura Red AC

Oil Red O certified by the Biological Stain Commission

Sigma-Aldrich

O0625

Oil Red O

composition

Dye content, 25%

composition

Dye content, 80%

composition

Dye content, ≥75%

composition

-

λmax

528 nm

λmax

504 nm

λmax

-

λmax

-

ε (extinction coefficient)

≥12000 at 277-283 nm in H2O, ≥15000 at 223-235 nm in H2O, ≥32000 at 524-530 nm in H2O

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Direct Red 80 (C.I. 35780) is an extremely hydrophilic, very large tetrakisazo hexasulfonated anionic dye that has a large, planar aromatic system.It is also known as Sirius red F3B, chlorantine fast red 7BLN or 5BRL, durazol brilliant red B, pontamine fast red 7BNL, and sirius fast red.

Application

Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.Other applications include -
  • detection of amyloid
  • Sirius red-Van Gieson methods for quantitation of collagen using image analysis methods
  • counterstaining applications substituting eosin in alum hematoxylins

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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