Direct Red 80 is not performance tested for use in a Picro Sirius Red staining method. Tissues fixed or stained with Picric Acid may require long rinse or wash steps to remove excess picric acid, and using slightly acidified water has been seen in protocols to remove the excess picric acid from tissue sections.
365548
Direct Red 80
Dye content 25 %
Synonym(s):
Sirius Red
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5 G
$50.30
25 G
$161.00
$50.30
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About This Item
Empirical Formula (Hill Notation):
C45H26N10Na6O21S6
CAS Number:
Molecular Weight:
1373.07
EC Number:
220-027-3
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
35780
Beilstein/REAXYS Number:
8185575
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InChI key
YIQKLZYTHXTDDT-IJPLDVMMSA-H
InChI
1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6/b50-48+,51-49+,54-52+,55-53+;;;;;;
SMILES string
[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c(cc4cc(NC(=O)Nc5ccc6c(O)c(\N=N\c7ccc(cc7S([O-])(=O)=O)\N=N\c8ccc(cc8)S([O-])(=O)=O)c(cc6c5)S([O-])(=O)=O)ccc14)S([O-])(=O)=O
form
powder
composition
Dye content, 25%
technique(s)
titration: suitable
color
dark brown
λmax
528 nm
ε (extinction coefficient)
≥12000 at 277-283 nm in H2O, ≥15000 at 223-235 nm in H2O, ≥32000 at 524-530 nm in H2O
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Related Categories
1 of 4
This Item | 195251 | 212490 | 458848 |
|---|---|---|---|
| technique(s) titration: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| color dark brown | color - | color - | color red to maroon |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form powder | form powder |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| composition Dye content, 25% | composition Dye content, 50% | composition Dye content, 30% | composition Dye content, 80% |
Application
Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.Other applications include -
- detection of amyloid
- Sirius red-Van Gieson methods for quantitation of collagen using image analysis methods
- counterstaining applications substituting eosin in alum hematoxylins
General description
Direct Red 80 (C.I. 35780) is an extremely hydrophilic, very large tetrakisazo hexasulfonated anionic dye that has a large, planar aromatic system.It is also known as Sirius red F3B, chlorantine fast red 7BLN or 5BRL, durazol brilliant red B, pontamine fast red 7BNL, and sirius fast red.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Extracellular matrix expansion is a key element of ventricular remodeling and a potential therapeutic target. Cardiovascular magnetic resonance (CMR) T1-mapping techniques are increasingly used to evaluate myocardial extracellular volume (ECV); however, the most widely applied methods are without histological validation.
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Using Direct Red 80 with Picric acid P6744 for Picro Sirius Red staining, and the staining resultss are coming out very yellow. Any advice on this issue?
1 answer-
Helpful?
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Is Direct Red 80 comparable to Dextran Red for back-filling injections in live zebrafish spinal cord?
1 answer-
Direct Red 80 is more commonly referred to as Sirius Red and is a stain for collagen. It is a vital stain used for various types of biological staining. Dextran Red is a fluorescent dye for which you would require a fluorescent microscope to view the staining. The different molecular weights of dextran are used to determine whether the dye crosses certain barriers.
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