Mayer's Mucicarmine Stain Solution is used to detect epithelial mucins and mucins secreting adendocarcinomonas and to demonstrate Cryptococcus neoformans and other fungi. The staining procedure involves placing a drop of the solution on a clean slide, then preparing and observing the stained fungal culture under a microscope.
41325
Mayer′s Mucicarmine Stain Solution
suitable for microscopy
Synonym(s):
Mucicarmine Stain Solution according to Mayer
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Product Name
Mayer′s Mucicarmine Stain Solution, suitable for microscopy
product line
BioChemika
form
liquid
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | staining: suitable
suitability
suitable for microscopy
fungi
application(s)
food and beverages
hematology
histology
storage temp.
room temp
Quality Level
1 of 4
This Item | 87794 | 43925 | 94635 |
|---|---|---|---|
| grade for microscopy | grade for microscopy | grade for microscopy | grade for microscopy |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| suitability fungi | suitability - | suitability Bacillus spp., Corynebacterium spp. | suitability - |
| application(s) food and beverages | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
General description
Most mucins contain serveral glycoproteins and can therefore be detected by stains for carbohydrates. Best carmine stain is specifically used for glycogens. Mucicarmine is an empirical stain. When applied for fungal staining, it stains the spores and hyphae as pale to dark red.
Other Notes
Composition:
Carmine 1 g, Aluminum chloride 0.5 g, water 2 ml
Carmine 1 g, Aluminum chloride 0.5 g, water 2 ml
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
supp_hazards
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Oluwole Fadare
Pathology international, 56(11), 683-687 (2006-10-17)
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Alexandra Flávia Gazzoni et al.
Mycopathologia, 167(4), 197-202 (2008-12-05)
Here we report an unusual case of disseminated cryptococcosis in a patient with AIDS. Although typical Cryptococcus neoformans micromorphology was observed in tongue biopsy, cervical lymph node examination revealed atypical histopathologic findings. These included pseudohyphae, chains of budding yeasts and
Ji Kon Ryu et al.
Diagnostic cytopathology, 31(2), 100-105 (2004-07-30)
Pancreatic cystic neoplasms comprise a pathologically heterogeneous group with many shared clinical features. We assessed the reliability of cyst fluid analysis for the differential diagnosis of pancreatic cysts. Cyst fluid was obtained by fine-needle aspiration from 78 pancreatic cysts. The
O E Battles et al.
American journal of clinical pathology, 108(1), 6-12 (1997-07-01)
To identify a sensitive marker for extramammary Paget's disease and to identify histochemical and immunohistochemical features that suggest occult pelvic cancer in patients with extramammary Paget's disease, we retrieved all cases between 1983 and 1992 with a Standardized Nomenclature of
Asim Qureshi
JPMA. The Journal of the Pakistan Medical Association, 57(3), 154-155 (2007-04-17)
A nephrectomy specimen was sent to the laboratory for end stage renal disease secondary to nephrolithiasis. Initial sections incidentally revealed a tumor infiltrating the normal renal tissue. Further workup including cytochemical and immuno-histochemical stains confirmed it to be collecting duct
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What is the Protocol for using Mayers Mucicarmine 41325 for staining fungi?
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Please provide the protocol for histology
1 answer-
Formulation 41325 contains carmine (1 g), aluminum chloride (0.5 g), and water (2 ml). On the other hand, the formulation for HT3018 consists of certified carmine (10 g/L), aluminum hydroxide (10 g/L), and aluminum chloride (5 g/L) in ethanol (50% w/v), following Southgate's modification of Mayer's mucicarmine procedure. Dilution instructions for use are provided, indicating the need to dilute product 41325 with either 10 volumes of distilled water or preferably 50-70% alcohol just before use.
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