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41782

Millipore

MRS Agar, Vegitone

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
DeMan, Rogosa and Sharpe Agar, Vegitone, Vegitone MRS Agar

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
ammonium citrate, 2.0 g/L
bromocresol green, 0.04 g/L
captan, 0.004 g/L
dextrose, 20.0 g/L
dipotassium phosphate, 2.0 g/L
magnesium sulfate, 0.1 g/L
manganese sulfate, 0.05 g/L
2-phenylethyl alcohol, 3.0 g/L
proteose peptone (vegetable), 10.0 g/L
sodium acetate, 5.0 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages

microbiology

suitability

selective and differential for Lactobacillus spp.
selective by low pH for Bifidobacterium spp.

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form

powder

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shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L , ammonium citrate, 2.0 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20.0 g/L , dipotassium phosphate, 2.0 g/L , magnesium sulfate, 0.1 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3.0 g/L , proteose peptone (vegetable), 10.0 g/L , sodium acetate, 5.0 g/L , yeast extract, 5.0 g/L

composition

ammonium citrate, 2 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20 g/L , dipotassium phosphate, 2 g/L , magnesium sulfate, 0.10 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3 g/L , proteose peptone (vegetable), 10 g/L , sodium acetate, 5 g/L , yeast extract, 5 g/L

composition

agar, 15.0 g/L , anilne blue, 0.1 g/L , lactose, 12.5 g/L , proteose peptone (vegetable), 5.0 g/L , sodium chloride, 5.0 g/L , synthetic detergent, 1.5 g/L , tryptose (vegetable), 10.0 g/L , yeast extract, 3.0 g/L

composition

agar, 20 g/L , ammonium sulfate, 1 g/L , calcium chloride, 0.02 g/L , L-cysteine hydrochloride, 1 g/L , dextrose, 5 g/L , magnesium sulfate, 0.2 g/L , monopotassium phosphate, 15 g/L , sodium chloride, 5 g/L , starch, soluble, 1 g/L , tryptone (vegetable), 4 g/L , tryptose (vegetable), 10 g/L , vegetable infusions powder, 10 g/L , yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

General description

This MRS Agar is free of animal derived material. It is recommended for the isolation and cultivation of Lactobacillus species.

Application

MRS Agar, Vegitone has been used for culturing yogurt to analyze lactic acid bacteria (LAB). It has also been used in culturing of Lactobacillus reuteri colonies.

Preparation Note

Suspend 62.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Cat. No. P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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He Ni et al.
Heliyon, 4(10), e00875-e00875 (2018-11-06)
In this study aqueous extracts from salal berry (SB) and blackcurrant pomace (BCP) were used to reformulate yogurt and the anti-diabetic properties of the beverage were investigated during 4 weeks of cold storage at 4 °C. Results indicated that α-amylase
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Nature communications, 9(1), 1822-1822 (2018-05-10)
Quorum sensing is a promising target for next-generation anti-infectives designed to address evolving bacterial drug resistance. The autoinducer-2 (AI-2) is a key quorum-sensing signal molecule which regulates bacterial group behaviors and is recognized by many Gram-negative and Gram-positive bacteria. Here

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