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432202

Sigma-Aldrich

Rhodamine 110 chloride

Dye content ≥75 %

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$118.00
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About This Item

Empirical Formula (Hill Notation):
C20H14N2O3 · HCl
CAS Number:
13558-31-1
Molecular Weight:
366.80
Beilstein/REAXYS Number:
4631860
EC Number:
236-944-7
MDL number:
MFCD00042009
UNSPSC Code:
12171500
PubChem Substance ID:
24867078
NACRES:
NA.47

Key Documents

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form

powder or crystals

Quality Level

200

composition

Dye content, ≥75%

mp

>300 °C (lit.)

λmax

496 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.Nc1ccc2c(OC3=CC(=N)C=CC3=C2c4ccccc4C(O)=O)c1

InChI

1S/C20H14N2O3.ClH/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;/h1-10,21H,22H2,(H,23,24);1H

InChI key

JNGRENQDBKMCCR-UHFFFAOYSA-N

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1 of 4

This Item
83695546062252433
Rhodamine 110 chloride Dye content ≥75 %

Sigma-Aldrich

432202

Rhodamine 110 chloride

Rhodamine 110 chloride suitable for fluorescence, BioReagent, ≥99.0% (UV)

Sigma-Aldrich

83695

Rhodamine 110 chloride

Rhodamine 19 perchlorate Dye content ~95 %

Sigma-Aldrich

546062

Rhodamine 19 perchlorate

Rhodamine 6G Dye content 99 %

Sigma-Aldrich

252433

Rhodamine 6G

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

form

powder or crystals

form

crystals

form

crystalline

form

solid

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

composition

Dye content, ≥75%

composition

-

composition

Dye content, ~95%

composition

Dye content, 99%

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

-

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

-

General description

Rhodamine 110 chloride (R110) is a laser grade dye.[1]

Application

Rhodamine 110 chloride has been used for the synthesis of the rhodamine 110 octadecyl ester (R110C18).[2]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK2166
BCCH6230
BCCG1785
BCCD6833
SHBM0519

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Aya Shibata et al.
Bioorganic & medicinal chemistry letters, 18(7), 2246-2249 (2008-03-25)
We have developed a new fluorescent probe for biological thiol. The probe was synthesized by the modification of the 2,4-dinitrobenzenesulfonyl group with rhodamine 110. The selective detection of thiol species such as cysteine or glutathione was achieved in biological conditions.
Michelle M Martinez et al.
Analytical and bioanalytical chemistry, 396(3), 1177-1185 (2009-11-26)
Early detection of apoptotic cells via caspase activity is demonstrated with fast response time. Fluorescence correlation spectroscopy (FCS) is used to identify the presence of a cleaved fluorogenic probe based on the fluorescence of rhodamine 110 in Jurkat cells. FCS
Sambashiva Banala et al.
ACS chemical biology, 7(2), 289-293 (2011-10-27)
A caged rhodamine 110 derivative for the specific labeling of SNAP-tag fusion proteins is introduced. The caged rhodamine 110 derivative permits the labeling of cell surface proteins in living cells and of intracellular proteins in fixed cells. The probe requires
Jaime G Mayoral et al.
Applied biochemistry and biotechnology, 160(1), 1-8 (2009-01-28)
A novel end-point fluorimetric procedure based on the use of rhodamine-110-labeled specific substrate was developed to determine trypsin activities in biological samples. We evaluated the ability of trichloroacetic acid and acetic acid to stop the enzymatic reaction without hindering the
Melissa M Yatzeck et al.
Bioorganic & medicinal chemistry letters, 18(22), 5864-5866 (2008-07-04)
A derivative of rhodamine 110 has been designed and assessed as a probe for cytochrome P450 activity. This probe is the first to utilize a 'trimethyl lock' that is triggered by cleavage of an ether bond. In vitro, fluorescence was
View All Related Papers

Questions

1–2 of 2 Questions  

  1. Is it okay to store Rhodamine 110 DMSO solution? If so, what is the temperature this solution should be store at?

    1 answer

    1. There is no data available regarding how solutions prepared in DMSO should be stored. The website does state in the Question section of the product detail page:

      It is not advised to store Rhodamine 110 solutions dissolved in water. Solutions must be used fresh. Solubility is better in DMSO, DMF, Methanol and Ethanol.

      A review of the sources Conn's Biological Stains, 10th Edition, The Sigma Aldrich Handbook of Stains, Dyes and Indicators by Floyd Green, and PubChem provided no information regarding solubility in DMSO or suggested storage. Based upon the fact that solutions in water are considered unstable, it is not recommended to store solutions of the compound.

      Helpful?

  2. What colour when soluble in water?

    1 answer

    1. Rhodamine 123 Choride is generally described as a green fluorescent dye. Excitation is around the 496-500 nm range. Excitation is around 520-522 nm. There is no specification for color once the dye is dissolved in a solvent.

      The colour of Rhodamine 110 Chloride in solid form is highly variable. The specification for 432202 states the color should be:
      Brown to Very Dark Brown and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown and Green to Very Dark Green.

      Rhodamine 110 chloride is only sparingly soluble in water. During quality control, product 432202 is dissolved in water at only 0.003 grams per liter. It is not advised to store Rhodamine 110 solutions dissolved in water. Solutions must be used fresh. Solubility is better in DMSO, DMF, Methanol and Ethanol.

      Helpful?

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