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Sigma-Aldrich

Nigrosin Stain solution

for microscopy

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CAS Number:
MDL number:
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

Bacillus spp.
Corynebacterium spp.

General description

Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.

Components

Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water

pictograms

Health hazardExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B - Skin Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jesse Schallek et al.
Investigative ophthalmology & visual science, 52(3), 1325-1335 (2010-11-06)
To examine the extent to which neurovascular coupling contributes to stimulus-evoked intrinsic signals in the retina. The retinas of five adult cats were examined in vivo. Animals were anesthetized and paralyzed for imaging stability. The retinas were imaged through a
K Matsuda et al.
Journal of clinical laboratory analysis, 15(4), 171-174 (2001-07-04)
We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 microl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining
J Moshonov et al.
Endodontics & dental traumatology, 11(5), 220-224 (1995-10-01)
The purpose of this study was to assess the efficacy of Nd:YAG laser irradiation in disinfecting the root canal system. Seventy-five teeth were uniformly instrumented and sterilized, and their root canals were infected for 60 min with an overnight culture
G E Freneau et al.
Animal reproduction science, 118(2-4), 176-181 (2009-09-30)
The objectives of this study were to compare two different methods of evaluating bull sperm morphology, bright-field (BF) microscopy of eosin-nigrosin (EN) stained dry-mount semen smears and differential interference phase contrast (DIC) microscopy of wet-mount semen 'fixed' in isotonic formal
Jie Guo et al.
Environmental science & technology, 42(2), 624-628 (2008-02-21)
Nonmetals reclaimed from waste phenolic cellulose paper printed circuit boards (PCBs) are used to replace wood flour in the production of phenolic molding compound (PMC). The results indicate that filling of nonmetals in PMC improves the charpy notched impact strength

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