46917

Supelco

9-Fluorenylmethyl carbazate

for HPLC derivatization, LiChropur, ≥99.0%

Synonym(s):
Fmoc-hydrazide
Empirical Formula (Hill Notation):
C15H14N2O2
CAS Number:
Molecular Weight:
254.28
Beilstein/REAXYS Number:
2470262
MDL number:
PubChem Substance ID:
NACRES:
NA.22

Quality Level

grade

for HPLC derivatization

assay

≥99.0% (HPLC)
≥99.0%

quality

LiChropur

application(s)

HPLC: suitable

mp

~170 °C (dec.)

fluorescence

λex 264 nm; λem 309 nm in methanol
λex 270 nm; λem 320 nm (Saccharide adducts)

storage temp.

2-8°C

SMILES string

NNC(=O)OCC1c2ccccc2-c3ccccc13

InChI

1S/C15H14N2O2/c16-17-15(18)19-9-14-12-7-3-1-5-10(12)11-6-2-4-8-13(11)14/h1-8,14H,9,16H2,(H,17,18)

InChI key

YGCGPEUVGHDMLO-UHFFFAOYSA-N

Application

9-Fluorenylmethyl carbazate may be used as a derivatization reagent to derivatize progesterone for high-performance liquid chromatography, in the analysis of progesterone, 17-hydroxyprogesterone and other 3-keto steroids.It may also be used as one of the fluorescence labeling agent for high-performance liquid chromatography determination of neutral and amino monosaccharides in glycoproteins by ultraviolet and fluorescence detection.

Other Notes

Reagent for the sensitive fluorogenic derivatization of carbohydrates

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Determination of progesterone and 17-hydroxyprogesterone by high performance liquid chromatography after pre-column derivatization with 4, 4-difluoro-5, 7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3-propionohydrazide.
Katayama M, et al.
Analyst, 123(11), 2339-2342 (1998)
Jinmao You et al.
Analytica chimica acta, 636(1), 95-104 (2009-02-24)
A pre-column derivatization method for the sensitive determination of aldehydes using the tagging reagent 2-[2-(7H-dibenzo[a,g] carbazol-7-yl)-ethoxy] ethyl carbonylhydrazine (DBCEEC) followed by high-performance liquid chromatography with fluorescence detection and APCI-MS identification has been developed. The chromophore of fluoren-9-methoxy-carbonylhydrazine (Fmoc-hydrazine) reagent was...
Journal of Chromatography A, 646, 45-45 (1993)
High-performance liquid chromatographic determination of neutral and amino monosaccharides by ultraviolet and fluorescence detection of sugar 9-fluoroenylmethoxycarbonyl hydrazones and 9-fluoroenylmethoxycarbonyl amino sugars at picomole and sub-picomole levels.
Zhang R, et al.
Journal of Chromatography A, 646(1), 45-52 (1993)
D R Rooyakkers et al.
Journal of chromatography. A, 730(1-2), 99-105 (1996-04-12)
Enteral intake of a mixture of inert, non-metabolic monosaccharide and disaccharide probes, followed by measurement of their urinary probe ratio, is a well known method to investigate gut permeability. However, most applications lack sensitivity, thus a large amount of especially...

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