Bile esculin agar

Name: Bile esculin agar
Brand: SIAL
Price: 270 USD
Availability: InStock

NutriSelect^® Plus, according to ISO 10273, powder, selective for Enterococcus spp., pack of 500 g

Synonym(s):

Esculin Bile Agar

Slide 1 of 2

Select a Size

500 G

$270.00

Estimated to ship onMarch 09, 2026Details

One Time Order

Request a Bulk Order

Subscriptions are currently unavailable.

About This Item

NACRES:

NA.74

UNSPSC Code:

41171606

Manufacturer/tradename:

NutriSelect^® Plus

Agency:

according to ISO 10273

Form:

powder

Suitability:

Streptococcus spp., selective for Enterococcus spp.

Application(s):

environmental
food and beverages
microbiology
water monitoring

Key Documents

View All Documentation

sterility

non-sterile

product line

BioChemika

form

powder

Quality Level

200

agency

according to ISO 10273

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L , esculin, 1 g/L , ferric citrate, 0.5 g/L , meat extract, 3 g/L , meat peptone, 5 g/L , ox-bile, 40 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect^® Plus

technique(s)

microbe id | specific enzyme detection: suitable

final pH

6.6±0.2 (25 °C)

application(s)

environmental
food and beverages
microbiology
water monitoring

suitability

Streptococcus spp., selective for Enterococcus spp.

Related Categories

Industrial Microbiology

Microbial Culture Media

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	72678	82981	92435
Millipore 48300 Bile esculin agar	Millipore 72678 Bile esculin azide agar ISO 7899-2:2000	Millipore 82981 Esculin Iron Agar	Millipore 92435 TBX Agar
technique(s) microbe id \| specific enzyme detection: suitable	technique(s) microbe id \| specific enzyme detection: suitable	technique(s) microbe id \| specific enzyme detection: suitable, microbiological culture: suitable	technique(s) -
form powder	form powder	form powder	form powder
suitability Streptococcus spp., selective and differential for Enterococcus spp.	suitability Enterococcus spp., Streptococcus spp.	suitability Streptococcus spp., nonselective and differential for Enterococcus spp.	suitability selective and differential for Escherichia coli, selective and differential for coliforms
agency according to ISO 10273:2017	agency according to ISO 7899-2:2000	agency -	agency according to ISO 16649-1:2018, according to ISO 16649-2:2001, according to ISO 16649-3:2015
composition agar, 15.0 g/L , ferric citrate, 0.5 g/L , meat peptone, 5 g/L , esculin, 1 g/L , ox-bile, 40 g/L , meat extract, 3 g/L	composition bacteriological agar, 15.0 g/L , esculin, 1.0 g/L , ferric ammonium citrate, 0.5 g/L , ox bile, 10.0 g/L , peptone, 3.0 g/L , sodium azide, 0.15 g/L , sodium chloride, 5.0 g/L , tyrptone, 17.0 g/L , yeast extract, 5.0 g/L	composition agar, 15 g/L , esculin, 1 g/L , ferric ammonium citrate, 0.5 g/L	composition agar, 15 g/L , bile salts, 1.5 g/L , X-β-D-glucuronide, 0.075 g/L , peptone, 20 g/L
sterility non-sterile	sterility non-sterile	sterility non-sterile	sterility non-sterile

Application

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.

For the preliminary identification of enterococci (serology group D), as defined by Swan (1954) and Facklam and Moody (1970).

Preparation Note

Dissolve 64.5 g in 1 litre distilled water. Bring to a boil while stirring continually. This medium should NOT be autoclaved. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.

Lindell, SHIRLEY S., and P. A. T. R. I. C. I. A. Quinn

Journal of Clinical Microbiology, 1 (5), 440-443 (1975)

Presumptive identification of group D streptococci: the bile-esculin test.

R R Facklam et al.

Applied microbiology, 20(2), 245-250 (1970-08-01)

Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for

Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation. Microbiology notes. Pratiksha Pokhrel September 1, 2015 Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation2015-10-16T17:40:41+00:00 Bacteriology, Biochemical Test, Diagnosis, Laboratory.

Science (2015)

The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).

A SWAN

Journal of clinical pathology, 7(2), 160-163 (1954-05-01)

Articles

Microbiological Control of Spices and Herbs

Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service