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48724

Millipore

Gelatin from porcine skin

high gel strength, suitable for microbiology

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CAS Number:
EC Number:
MDL number:
NACRES:
NA.85

biological source

Porcine

Quality Level

sterility

non-sterile

form

powder

quality

high gel strength

shelf life

limited shelf life, expiry date on the label

ign. residue

≤2%

loss

≤15% loss on drying

transmittance

450 nm, ≥85%
620 nm, ≥95%

pH

4.0-6.0 (25 °C, 67 mg/mL in H2O)
5.20-5.60

viscosity

5.10-5.80 mPa.s

gel strength

240-270 g Bloom (67 mg/ml water)
280-320 g BloomAOAC

solubility

H2O: 67 mg/mL at 50 °C, slightly hazy, faintly yellow
H2O: 67 mg/mL at 60 °C

anion traces

chloride (Cl-): ≤2000 mg/kg

cation traces

Ca: ≤2000 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤10 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤500 mg/kg
Mg: ≤500 mg/kg
Mn: ≤5 mg/kg
Na: ≤1200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤10 mg/kg

application(s)

microbiology

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This Item
G2500G6144G9136
Gelatin from porcine skin high gel strength, suitable for microbiology

48724

Gelatin from porcine skin

Gelatin from porcine skin gel strength 300, Type A

G2500

Gelatin from porcine skin

Gelatin from porcine skin gel strength 80-120 g Bloom, Type A

G6144

Gelatin from porcine skin

Sigma-Aldrich

G9136

Gelatin from porcine skin

application(s)

microbiology

application(s)

-

application(s)

-

application(s)

-

biological source

Porcine

biological source

Porcine skin

biological source

-

biological source

Porcine skin

sterility

non-sterile

sterility

-

sterility

-

sterility

γ-irradiated

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

solubility

H2O: 67 mg/mL at 50 °C, slightly hazy, faintly yellow, H2O: 67 mg/mL at 60 °C

solubility

H2O: soluble 50 mg/mL

solubility

-

solubility

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 250. Manufactured by Gelita AG

Storage Class

11 - Combustible Solids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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