49940

ONPG Disks

suitable for microbiology

• Synonym(s): β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks
• UNSPSC Code: 41171621
• NACRES: NA.85

Properties

• agency: according to GB 4789.4-2016
• product line: BioChemika
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | specific enzyme detection: suitable
• application(s): environmental; food and beverages; microbiology
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: Escherichia coli; bacteria

Description

General description

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Packaging

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)