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4B200

Sigma-Aldrich

Sepharose 4B

45-165 μm bead diameter

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CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

biological source

algae (marine)

Quality Level

form

suspension

technique(s)

affinity chromatography: suitable

bead diameter

45-165 μm

pore size

30,000-50,000 Da fractionation range (Dextrans)
60,000-20,000,000 Da fractionation range (Globular proteins)

storage temp.

2-8°C

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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1 of 4

This Item
GE17-0120-016B100GE17-0110-01
Sepharose™ 4B 45-165 μm bead diameter

Sigma-Aldrich

4B200

Sepharose 4B

Sepharose™ 4B Cytiva 17-0120-01, pack of 1 L

GE17-0120-01

Sepharose 4B

Sepharose™ 6B 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)

Sigma-Aldrich

6B100

Sepharose 6B

Sepharose™ 6B Cytiva 17-0110-01, pack of 1 L

GE17-0110-01

Sepharose 6B

form

suspension

form

-

form

suspension

form

-

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

bead diameter

45-165 μm

bead diameter

-

bead diameter

-

bead diameter

-

General description

Sepharose 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Application

Sepharose 4B has been used:
  • to conjugate scorpion venom to be used in affinity chromatography
  • for clearing of protein extracts prior to immunoprecipitation
  • for gel purification and desalting of phospholipid vesicles
  • for adsorption of immunoglobulin G (IgG)

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F - closed cup

Flash Point(C)

38 - 43 °C - closed cup


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