50875

Millipore

GSP Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):
Pseudomonas Aeromonas Selective Agar acc. to Kielwein
NACRES:
NA.74

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
magnesium sulfate, 0.5 g/L
phenol red, 0.36 g/L
potassium dihydrogen phosphate, 2 g/L
sodium L-glutamate, 10 g/L
starch (soluble), 20 g/L

manufacturer/tradename

NutriSelect® Plus

application(s)

bioburden testing: suitable
microbiological culture: suitable
microbiology: suitable
water monitoring: suitable

final pH

7.2±0.2 (25 °C)

Featured Industry

Environmental
Food and Beverages

suitability

Aeromonas spp.
selective and differential for Pseudomonas spp.

Related Categories

General description

Agar is a complex gelatinous carbohydrate, and is added to the LB broth (Lysogeny broth, Luria broth, Lennox broth, or Luria-Bertani medium), in order to form gel for bacteria to grow upon. LB Broth is the most widely used nutritionally rich medium, for the culture and growth of bacteria. There are several different formulations of Lb broth, but the composition is generally the same comprising of peptides and casein peptones, trace elements, mineral and vitamins.

Application

For the detection of Pseudomonas and Aeromonas in all types of food as well as in the equipment and water supply of food-processing firms.

Preparation Note

Dissolve 45 g in 1 litre distilled water and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 100′000 IU/l Penicillin G (Cat. No. 13752; 60mg/l) and if desired 10 mg/l Pimaricin (Cat. No. 80482; 400ul/l). Mix well and pour into sterile petri plates.

Other Notes

A medium for selective cultivation of Pseudomonas and Aeromonas; The aerobic Pseudomonas - a taxonomic study

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
G. Kielwein
Archiv Fur Lebensmittelhygiene, 20, 131-131 (1969)
R.Y. Stanier et al.
Journal of General Microbiology, 42, 159-159 (1966)
Archiv Fur Lebensmittelhygiene, 22, 15-15 (1971)
Agar and agarose biotechnological applications.
Armisen R.
Hydrobiologia, 221 (1), 157-166 (1991)
The structure of agar: Part I. Fractionation of a complex mixture of polysaccharides.
Duckworth M and Yaphe W.
Carbohydrate Research, 16 (1), 189-197 (1971)

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