53286

Brain Heart Infusion Broth

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): BHI Broth, Brain Heart Broth
• NACRES: NA.74

Properties

• Agency: according to ISO 6888-1 2020; according to ISO 6888-3:2003
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: beef heart (infusion from 250g), 5 g/L ; calf brains (infusion from 200g), 12.5 g/L ; disodium hydrogen phosphate, 2.5 g/L ; D(+)-glucose, 2 g/L ; peptone, 10 g/L ; sodium chloride, 5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; life science and biopharma; pathogen testing; pharmaceutical; microbiology
• suitability: Haemophilus spp.; nonselective for Bacteroides spp.; nonselective for Candida spp.; nonselective for Escherichia coli; nonselective for Neisseria spp.; nonselective for Pseudomonas spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for Yersinia spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

Application

Brain Heart Infusion Broth is recommended for the cultivation of fastidious pathogenic microorganisms. Brain Heart Infusion Broth has been used as culture medium forStreptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes and Clostridium difficile.

Preparation Note

Dissolve 37 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Production of 12α-hydroxysteroid dehydrogenase from Clostridium group P

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves