53286

Brain Heart Infusion Broth

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): BHI Broth, Brain Heart Broth, BHI Broth, Brain Heart Broth
• NACRES: NA.74

Properties

• Agency: according to ISO 6888-1 2020; according to ISO 6888-3:2003
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: beef heart (infusion from 250g), 5 g/L ; calf brains (infusion from 200g), 12.5 g/L ; disodium hydrogen phosphate, 2.5 g/L ; D(+)-glucose, 2 g/L ; peptone, 10 g/L ; sodium chloride, 5 g/L
• packaging: pkg of 100 g; pkg of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: light yellow
• final pH: 7.4±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; life science and biopharma; pathogen testing; pharmaceutical; microbiology
• suitability: Haemophilus spp.; nonselective for Bacteroides spp.; nonselective for Candida spp.; nonselective for Escherichia coli; nonselective for Neisseria spp.; nonselective for Pseudomonas spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for Yersinia spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Brain Heart Infusion Broth is recommended for the cultivation of various fastidious, pathogenic microorganisms, including yeasts and molds. The media is nutritious and well-buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. The media is especially suited for the cultivation of Staphylococci for the plasma coagulase test and setting up blood cultures. The addition of ascites permits the cultivation of Gonococci. The agar improves the growth of microaerophilic and anaerobic microorganisms. With the addition of 10% defibrinated sheep blood, it is suitable for the isolation and cultivation of Histoplasma capsulatum and other pathogenic fungi. Enzymatic digestion of animal tissues and brain-heart infusion provide amino acids, nitrogen, carbon, vitamins, and minerals for the growth of organisms. Glucose is the source of carbohydrates, and sodium chloride maintains the osmotic balance of the medium. Disodium phosphate is a buffering agent.

Application

Brain Heart Infusion Broth is recommended for the cultivation of fastidious pathogenic microorganisms and is often used for the examination of water, wastewater, meat, and food products. It is used as a culture medium forStreptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes and Clostridium difficile.

Features and Benefits

NutriSelect^® Plus are powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Dissolve 37 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Production of 12α-hydroxysteroid dehydrogenase from Clostridium group P

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves