Brain Heart Infusion Broth

suitable for microbiology, NutriSelect® Plus

BHI Broth, Brain Heart Broth

Quality Level


according to ISO 6888-1 2020
according to ISO 6888-3:2003





shelf life

limited shelf life, expiry date on the label


beef heart (infusion from 250g), 5 g/L
calf brains (infusion from 200g), 12.5 g/L
disodium hydrogen phosphate, 2.5 g/L
D(+)-glucose, 2 g/L
peptone, 10 g/L
sodium chloride, 5 g/L


NutriSelect® Plus


microbiological culture: suitable
microbiology: suitable
pathogen testing: suitable

final pH

7.4±0.2 (25 °C)

Featured Industry

Clinical Testing
Food and Beverages
Life Science and Biopharma


Haemophilus spp.
nonselective for Bacteroides spp.
nonselective for Candida spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for Yersinia spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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Brain Heart Infusion Broth is recommended for the cultivation of fastidious pathogenic microorganisms. Brain Heart Infusion Broth has been used as culture medium forStreptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes and Clostridium difficile.

Preparation Note

Dissolve 37 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Production of 12α-hydroxysteroid dehydrogenase from Clostridium group P

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Economic evaluation of Streptococcus pneumoniae culture media
Morais V and Suarez N
American Journal of Biochemistry and Biotechnology, 12, 133-138 (2016)
Comparison of culture media in the isolation and diagnosis of cutaneous leishmaniasis
Ahmet O, et al.
African Journal of Microbiology Research, 4(11), 1038-1043 (2010)
Gene expression profiling of Listeria monocytogenes strain F2365 during growth in ultrahigh-temperature-processed skim milk
Liu Y and Ream A
Applied and Environmental Microbiology, 74(22), 6859-6866 (2008)
Liang Li et al.
mBio, 10(3) (2019-06-06)
Secondary bacterial lung infection by Streptococcus pneumoniae (S. pneumoniae) poses a serious health concern, especially in developing countries. We posit that the emergence of multiantibiotic-resistant strains will jeopardize current treatments in these regions. Deaths arising from secondary infections are more...
Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism
Fachi J, et al.
Testing, 27(3), 750-761 (2019)
An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.
Read More
Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.
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Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
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