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54793

Supelco

2-(4-Hydroxyphenylazo)benzoic acid

matrix substance for MALDI-MS, ≥99.5%

Synonym(s):
HBABA, HABA, 4′-Hydroxyazobenzene-2-carboxylic acid, 2-(4′-Hydroxybenzeneazo)benzoic acid
Linear Formula:
HOC6H4N=NC6H4CO2H
CAS Number:
Molecular Weight:
242.23
Beilstein:
1842696
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

grade

matrix substance for MALDI-MS

assay

≥99.5% (HPLC)
≥99.5%

analyte functional class(es)

polymers

analyte chemical class(es)

glycans, peptides, proteins

application(s)

MALDI-MS: suitable

mp

204-208 °C (lit.)
204-208 °C

solubility

ethanol: 20 mg/mL, clear

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤50 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤100 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

absorption

≥2000 at 337 nm (mol. absorption)

SMILES string

Oc1ccc(cc1)\N=N\c2ccccc2C(O)=O

InChI

1S/C13H10N2O3/c16-10-7-5-9(6-8-10)14-15-12-4-2-1-3-11(12)13(17)18/h1-8,16H,(H,17,18)/b15-14+

InChI key

DWQOTEPNRWVUDA-CCEZHUSRSA-N

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General description

2-(4-Hydroxyphenylazo)benzoic acid) (HABA), can be used for desorption of proteins, peptides and glycoproteins, thereby making it very advantageous for matrix-assisted laser desorption ionization mass spectrometry.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

K J Welham et al.
Rapid communications in mass spectrometry : RCM, 12(4), 176-180 (1998-03-11)
A variety of gram-positive and gram-negative intact bacterial cells have been analysed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and shown to provide fingerprint mass spectra with discrete peaks being observed over the mass range from 3 to 40 kDa....
Matrix-assisted laser desorption ionization mass spectrometry with 2-(4-hydroxyphenylazo) benzoic acid matrix.
Juhasz, Peter, Catherine E. Costello, and Klaus Biemann.
Journal of the American Society For Mass Spectrometry, 4 (5), 399-409 (1993)
M A Domin et al.
Rapid communications in mass spectrometry : RCM, 13(4), 222-226 (1999-03-31)
The ability to rapidly identify the taxonomic class of the wide variety of microorganisms involved in human and animal disease is becoming increasingly important, especially with the increasing development of resistance to the antibiotics which form the main defence against...
Kai Qi et al.
Journal of the American Chemical Society, 126(21), 6599-6607 (2004-05-27)
Shell cross-linked nanoparticles (SCKs) presenting surface- and bioavailable biotin functional groups were synthesized via a mixed micelle methodology, whereby co-micellization of chain terminal biotinylated poly(acrylic acid)-b-poly(methyl acrylate) (PAA-b-PMA) and nonbiotinylated PAA-b-PMA were cross-linked in an intramicellar fashion within the shell...
Noritaka Kato et al.
The journal of physical chemistry. B, 109(42), 19604-19612 (2006-07-21)
This study reports a homogeneous and competitive fluorescence quenching immunoassay based on gold nanoparticle/polyelectrolyte (Au(NP)/PE) coated latex particles prepared by the layer-by-layer (LbL) technique. First, the resonant energy transfer from a layer of fluorescent PEs to Au(NP) in LbL assembled...

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