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61135

Sigma-Aldrich

Crystal Violet

for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

Synonym(s):
Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
Linear Formula:
C25H30N3Cl
CAS Number:
Molecular Weight:
407.98
Colour Index Number:
42555
Beilstein:
3580948
MDL number:
PubChem Substance ID:
NACRES:
MA.02

grade

for microscopy (Bact., Bot., Hist., Vit.)
indicator (pH 0.1-2.0)

Quality Level

form

powder

visual transition interval

0.1-2.0, yellow-green to blue-violet

mp

205 °C (dec.) (lit.)

density

1.190 g/cm3

ε (extinction coefficient)

≥1750 at 585-595  nm in water

suitability

suitable for microscopy (Bact., Bot., Hist., Vit.)

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

Mode of action

cell membrane | interferes
enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

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This Item
C0775C077532675
Crystal Violet for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

Sigma-Aldrich

61135

Crystal Violet

Crystal Violet certified by the Biological Stain Commission

Sigma-Aldrich

C0775

Crystal Violet

Crystal Violet certified by the Biological Stain Commission

Sigma-Aldrich

C0775

Crystal Violet

Crystal Violet indicator for the determination of the redox potential, S. No.: 785

Sigma-Aldrich

32675

Crystal Violet

grade

for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

grade

certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

grade

indicator for the determination of the redox potential

form

powder

form

powder

form

powder

form

powder

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

mp

205 °C (dec.) (lit.)

density

1.190 g/cm3

density

1.19 g/cm3 at 20 °C

density

1.19 g/cm3 at 20 °C

density

1.190 g/cm3

ε (extinction coefficient)

≥1750 at 585-595  nm in water

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

suitability

suitable for microscopy (Bact., Bot., Hist., Vit.)

suitability

-

suitability

-

suitability

complies for sensitivity

General description

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It is a hexa-N-methylated triaminotriphenylmethane with a lipophilic cation. It possesses a slightly nonplanar conjugated system.

Application

Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for the staining of plant chromosomes.Crystal violet is also suitable for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal Violet also helps in the colorimetric measurement of cell viability.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Muta. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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