61135

Crystal Violet

for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

• Synonym(s): Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
• Linear Formula: C₂₅H₃₀N₃Cl
• CAS Number: 548-62-9
• Molecular Weight: 407.98
• Colour Index Number: 42555
• Beilstein: 3580948
• MDL number: MFCD00011750
• PubChem Substance ID: 329759365
• NACRES: MA.02

Properties

• grade: for microscopy (Bact., Bot., Hist., Vit.); indicator (pH 0.1-2.0)
• Quality Level: 200
• form: powder
• visual transition interval: 0.1-2.0, yellow-green to blue-violet
• mp: 205 °C (dec.) (lit.)
• density: 1.190 g/cm³
• ε (extinction coefficient): ≥1750 at 585-595 nm in water
• suitability: suitable for microscopy (Bact., Bot., Hist., Vit.)
• antibiotic activity spectrum: fungi
• application(s): diagnostic assay manufacturing; hematology; histology
• Mode of action: cell membrane | interferes; enzyme | inhibits
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChI key: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

General description

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It is a hexa-N-methylated triaminotriphenylmethane with a lipophilic cation. It possesses a slightly nonplanar conjugated system.

Application

Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for the staining of plant chromosomes.Crystal violet is also suitable for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal Violet also helps in the colorimetric measurement of cell viability.

Safety Information

• Pictograms: GHS05,GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H318 - H341 - H350 - H410
• Precautionary Statements: P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Muta. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves